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SD大鼠心脏局部照射放射性心肌纤维化模型建立
姚晔,陈晶,李珍珍,李鑫,程静,杨洪娟,钟亚华,谢丛华
430071 武汉大学中南医院放化疗科肿瘤生物学行为湖北省重点实验室
Establishment and validation of radiation-induced heart fibrosis on SD rat model
Yao Ye,Chen Jing,Li Zhenzhen,Li Xin,Chen Jing,Yang Hongjuan,Zhong Yahua,Xie Conghua
Department of Radiation Oncology and Medical Oncology,Zhongnan Hospital ofWuhan University,Hubei Cancer Clinical Study Center,Hubei Key Laboratory of Tumor Biological Behaviors,Wuhan 430071,China
Abstract：Objective To establish a Sprague-Dawley (SD) rat model of radiation-induced heart injury and myocardial fibrosis. Methods Using the small animal radiation research platform,18-Gy radiation was given to the heart of male SD rats in a single fraction. At six months after radiation,heart structure and function were assessed by color Doppler ultrasound;heart injury and collagen fiber deposition were evaluated using HE staining and picrosirius red staining,respectively;heart collagen content was analyzed by hydroxyproline measurement. Results According to the color Doppler ultrasound assessment,the left ventricular posterior wall thickness during systole was significantly smaller in the radiation group than in the control group (0.22±0.01 vs. 0.25±0.05 cm,P=0.037).The results of picrosirius red staining showed that the proportions of fibrosis area in the right ventricle,interventricular septum,and left ventricle were significantly higher in the radiation group than in the control group ((2.99±1.29)% vs. (2.21±0.87)%,P=0.025;(2.53±1.27)% vs. (0.91±0.70)%,P=0.000;(2.45±1.98)% vs. (0.54±0.31)%,P=0.000). According to the measurement of hydroxyproline concentration,the radiation group had a significantly higher level of hydroxyproline than the control group (0.56±0.02 vs. 0.49±0.05 ug/mg,P=0.010),indicating severe heart collagen deposition after radiation. Conclusions At the end of model establishment,SD rats have obvious myocardial fibrosis and reduced left ventricular posterior wall thickness during systole,suggesting that a SD rat model of radiation-induced heart injury and myocardial fibrosis is successfully established.
Yao Ye,Chen Jing,Li Zhenzhen et al. Establishment and validation of radiation-induced heart fibrosis on SD rat model[J]. Chinese Journal of Radiation Oncology, 2017, 26(1): 84-89.
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