Immediate early response gene 5 in cervical cancer tissues:radiosensitivity and clinical significance
Liu Yang,Wu Yumei,Tian Ming,Zhao Hui,Yu Xinping,Zhou Pingkun
Department of Gynecological Oncology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100006,China (Liu Y,Wu YM,Tian M,Zhao H,Yu XP); Department of Radiation Toxicology and Oncology,Beijing Institute of Radiation Medicine,Beijing 100850,China (Zhou PK)
Abstract： Objective To investigate the expression features of immediate early response gene 5(IER5) in patients with cervical cancer before and after radiotherapy,and to study the relationship of IER5 with radiosensitivity and clinical outcomes. Methods From 2014 to 2015,39 patients with stage Ⅱb-Ⅲb cervical cancer undergoing concurrent chemoradiotherapy were enrolled as subjects. Cervical cancer tissues were collected before and after radiotherapy with doses of 2-6,10,20,and 30 Gy. Western blotting was used to determine the relative expression of IER5 protein. Results The expression of IER5 protein in cervical cancer tissues was significantly higher after exposure to 10,20,and 30 Gy of radiation (t=3.06,4.01,6.99,P<0.01).The expression of IER5 protein was correlated with the radiation dose (r=0.511,P<0.01).There was a significant interaction between the expression of IER5 protein under different doses of irradiation and the tumor size before radiotherapy (F=3.212,P<0.05).When the tumor diameter was<4 or 4-5 cm before treatment,the expression of IER5 had significant radiation dose-dependent variation (F=10.493,P<0.01;F=9.977,P<0.01).However,there was no significant dose-dependent variation in the expression of IER5 protein when the tumor diameter was>5 cm. Conclusions The expression of IER5 protein in patients with cervical cancer is significantly higher after radiotherapy. Moreover,IER5 expression gets higher with the increase in radiation dose,indicating that IER5 is related to the radiosensitivity of patients with cervical cancer. The smaller the tumor,the more radiation dose-dependent the IER5 expression.
Liu Yang,Wu Yumei,Tian Ming et al. Immediate early response gene 5 in cervical cancer tissues:radiosensitivity and clinical significance[J]. Chinese Journal of Radiation Oncology, 2016, 25(12): 1345-1349.
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