Abstract：Objective To investigate the reliable Methods for establishing models of acute radiation enteritis (ARE) and the criteria used to judge whether the model is successfully established. Methods A total of 98 rats were randomly divided into normal control group (group A), fractionated dose group B (4 Gy/fraction for 3 fractions), fractionated dose group C (4 Gy/fraction for 4 fractions), fractionated dose group D (4 Gy/fraction for 5 fractions), single fraction group E (12 Gy in a single fraction), single fraction group F (16 Gy in a single fraction), and single fraction group G (20 Gy in a single fraction). Abdominal irradiation was performed for all rats, and the changes in body weight and defecation were observed. Magnetic resonance imaging (MRI) was performed on days 3-5 after irradiation, and on the 4th day, anatomy was performed to measure the length of small intestine with edema, blood samples were collected to measure endotoxins, and the specimens of small intestine were collected to observe pathological changes. The independent-samples t-test was used for comparison between groups. Results After irradiation, groups D, E, F, and G experienced varying degrees of diarrhea and had positive results from endotoxins test. Group D had a longer length of small intestine with edema than group C (P=0.00) and had a similar length as group E (P=0.46). Groups E, F, and G showed dilation and dropsy in the intestinal canal on MRI, and groups F and G showed patchy signals of dropsy in the abdominal cavity. Groups F and G showed varying degrees of necrosis in the small intestine and died within 14 days after irradiation. Conclusions When the radiation dose is 33-46 Gy (biologically equivalent dose), both single dose and fractionated dose can successfully establish the model of ARE, while fractionated dose can be better controlled.
Wang Yingjie,Wang Shunjin,Gong Lianggeng et al. Models of acute radiation enteritis:a comparison and evaluation of two modeling methods[J]. Chinese Journal of Radiation Oncology, 2016, 25(6): 628-633.
[1] Matthews MA,Watkins D,Darbyshire A,et al Heparin-binding EGF-like growth factor (HB-EGF) protectsthe intestines from radiation therapy-induced intestinal injury[J]. J Pediatr Surg,2013,48(6):1316-1322.DOI:10.1016/j.jpedsurg.2013.03.030. [2] Stacey R,Green JT. Radiation-induced small bowel disease:latest developments and clinical guidance[J]. Ther Adv Chronic Dis,2014,5(1):15-29. DOI:10.1177/2040622313510730. [3] Wang TJ,Liu ZS,Zeng ZC,et al. Caffeine does not enhance radiosensitivity of normal liver tissue in vivo[J]. Mol Biol Rep,2011,38(7):4359-4367. DOI:10.1007/s11033-010-0563-7. [4] Cameron S,Schwartz A,Sultan S,et al. Radiation-induced damage in different segments of the rat intestine after external beam irradiation of the liver[J]. Exp Mol Pathol,2012,92(2):243-258.DOI:10.1016/j.yexmp.2011.11.007. [5] Chu J,Zhang X,Jin L,et al. Protective effects of caffeic acid phenethyl ester against acute radiation-induced hepatic injury in rats[J]. Environ Toxicol Pharmacol,2015,39(2):683-689. DOI:10.1016/j.etap.2015.01.020. [6] Kanter M,Topcu-Tarladacalisir Y,Uzal C. Role of amifostine on acute and late radiation nephrotoxicity:a histopathological study[J]. In Vivo,2011,25(1):77-85. [7] Kucuktulu E. Protective effect of melatonin against radiation induced nephrotoxicity in rats[J]. Asian Pac J Cancer Prev,2012,13(8):4101-4105.DOI:http://dx.doi.org/10.7314/APJCP.2012.13.8.4101. [8] 阁皓,王辉,张恒,等.白藜芦醇对急性辐射损伤小鼠的防护作用[J]. 中华放射医学与防护杂志,2014,34(7):512-515.DOI:10.3760/cma.j.issn.0254-5098.2014.07.008. Ge G,Wang H,Zhang H,et al. Protective effects of resveratrol on acute radiation injury in mice[J].Chin J Raiol Med Prot,2014,34(7):512-515.DOI:10.3760/cma.j.issn.0254-5098.2014.07.008. [9] 王瑜,张再重,陈少全,等.N-乙酰半胱氨酸对大鼠辐射损伤相关肠屏障功能障碍的防护作用[J]. 中华胃肠外科杂志,2010,13(3):219-222.DOI:10.3760/cma.j.issn.1671-0274.2010.03.016. Wang Y,Zhang ZCH,Chen SHQ,et al. Protective effect of N— N-acetyl cysteine on intestinal barrier dysfunction associated with radiation injury in rats[J].Chin J Gastrointest Surg,2010,13(3):219-222.DOI:10.3760/cma.j.issn.1671-0274.2010.03.016. [10] 常鹏宇,姜新,曲超,等.脂肪干细胞修复放射性肠上皮损伤的实验研究[J]. 中华放射医学与防护杂志,2014,34(4):271-274. DOI:10.3760/cma.j.issn.0254-5098.2014.04.008. Chang PY,Jiang X,Qu CH,et al. Experimental study on the repair of radiation-induced intestinal epithelial injury by adipose derived stem cells[J].Chin J Raiol Med Prot,2014,34(4):271-274. DOI:10.3760/cma.j.issn.0254-5098.2014.04.008. [11] Jang SY,Park JW,Bu Y. Protective effects of hominis placenta hydrolysates on radiation enteropathy in mice[J]. Nat Prod Res,2011,25(20):1988-1992.DOI:10.1080/14786419.2010.513035. [12] Driák D,Osterreicher J,Reháková Z,et al. Expression of phospho-Elk-1 in rat gut after the whole body gamma irradiation[J]. Physiol Res,2008,57(5):753-759. [13] 杜傲男,徐静,何燕,等.神经源性分化因子对小鼠放射性肠损伤的治疗作用[J]. 中华放射医学与防护杂志,2015,35(1):45-48. DOI:10.3760/cma.j.issn.0254-5098.2015.01.008. Du AN,Xu J,He Y,et al. The therapeutic effect of neurogenic differentiation factor on radiation-induced intestinal injury in mice[J].Chin J Raiol Med Prot,2015,35(1):45-48. DOI:10.3760/cma.j.issn.0254-5098.2015.01.008. [14] Karliczek A,Zeebregts CJ,Benaron DA,et al. Preoperative irradiation with 5 x 5 Gy in a murine isolated colon loop model does not cause anastomotic weakening after colon resection[J]. Int J Colorectal Dis,2008,23(11):1115-1124. DOI:10.1007/s00384-008-0507-z. [15] Gremy O,Benderitter M,Linard C. Acute and persisting Th2-like immune response after fractionated colorectal gamma-irradiation[J]. World J Gastroenterol,2008,14(46):7075-7085.DIO:10.3748/wjg.14.7075. [16] Wang R,Yuan W,Zhao Q,et al. An experimental study of preventing and treating acute radioactive enteritis with human umbilical cord mesenchymal stem cells[J]. Asian Pac J Trop Med,2013,6(12):968-971. DOI:10.1016/S1995-7645(13)60173-X. [17] Song S,Chen D,Ma T,et al. Molecular mechanism of acute radiation enteritis revealed using proteomics and biological signaling network analysis in rats[J]. Dig Dis Sci,2014,59(11):2704-2713. DOI:10.1007/s10620-014-3224-1. [18] Toklu HZ,Sehirli O,Ozyurt H,et al. Punica granatum peel extract protects against ionizing radiation-induced enteritis and leukocyte apoptosis in rats[J]. J Radiat Res,2009,50(4):345-353.DOI:10.1269/jrr.08126. [19] Rubio CA,Jalnas M. Dose-time-dependent histological changes following irradiation of the small intestine of rats[J]. Dig Dis Sci,1996,41(2):392-401. [20] 于涛,石柳,邓娜,等.肠上皮干细胞标志分子在小鼠放射性肠炎中的表达[J].中华消化杂志,2011,31(5):325-329. DOI:10.3760/cma.j.issn.0254-1432.2011.05.010. Yu T,Shi L,Deng N,et al. Expression of intestinal epithelial stem cell marker in mice with radiation enteritis[J].Chin J Digest,2011,31(5):325-329. DOI:10.3760/cma.j.issn.0254-1432.2011.05.010. [21] Xu W,Chen J,Xu L,et al. Acute radiation enteritis caused by dose-dependent radiation exposure in dogs:experimental research[J]. Exp biol Med,2014,239(12):1543-1556. DOI:10.1177/1535370214533884. [22] 洪智攀,武寒飞,赵克,等.不同照射剂量下大鼠急性放射性肠炎模型的建立与比较[J]. 中华实验外科杂志,2012,29(11):2317-2320. DOI:10.3760/cma.j.issn.1001-9030.2012.11.077. Hong ZHP,Wu HF,Zhao K,et al. Establishment and comparison of acute radiation enteritis model in rats under different irradiation doses[J].Chin J Experiment Surg,2012,29(11):2317-2320. DOI:10.3760/cma.j.issn.1001-9030.2012.11.077. [23] 徐伟,金晶.直线加速器诱发大鼠急性放射性肠炎模型的建立[J]. 实用临床医药杂志,2013,17(11):9-11. DOI:10.7619/jcmp.201311003. Xu W,Jin J.Establishment of acute radiation enteritis model in rats induced by linear accelerator[J].J Pract Clin Med,2013,17(11):9-11.DOI:10.7619/jcmp.201311003. [24] 王玉,周冬枝,夏欣欣,等.大黄素对急性放射性肠炎肠黏膜屏障的保护作用[J]. 西安交通大学学报(医学版),2013,34(2):248-252. Wang Y,Zhou DZH,Xia XX,et al. Protective effect of emodin on intestinal mucosal barrier in acute radiation enteritis[J].J Xi′an Jiaotong Univ (Med Sci Ed),2013,34(2):248-252. [25] AbbasoluSD,Erbil Y,Eren T,et al. The effect of heme oxygenase-1 induction by octreotide on radiation enteritis[J].Peptides,2006,27(6):1570-1576.DOI:10.1016/j.peptides.2005.11.012.
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