Radiosensitizing effect and mechanism of 2′-hydroxyflavanone in prostate cancer cells
Wang Wen,Xiong Wei,Li Xiaoying,Gao Xianshu,Sun Shaoqian,Li Yi
Department of Radiation Oncology,First Hospital,Peking University,Beijing 100034,China (Wang W,Li XY,Gao XSH,Sun SHQ);Department of Radiotherapy and Chemotherapy,Tangshan City People s Hospital,Tangshan 063001,China;School of Pharmacy,Peking University,Beijing 100191,China (Li Y)
Abstract： Objective To study the radiosensitizing effect of 2′-hydroxyflavanone (2′-HF) on prostate cancer cells, and to preliminarily investigate its mechanism. Methods Colony formation assay, tert-butylhydroperoxide (TBHP) oxidative stress assay, Hoechst staining, and apoptosis flow cytometry using Annexin V-FITC and propidium iodide (PI) were performed to measure the impact of 2′-HF on the radiosensitivity of VCaP prostate cancer cells. Western blot was used to determine the effects of 2′-HF on expression of AKT, phosphorylated AKT (p-AKT), and aldo-keto reductase 1C3(AKR1C3) in VCaP cells and preliminarily investigate the mechanism. Data were analyzed by t test and factorial analysis of variance. Results The results of colony formation assay indicated that after exposure to radiation, VCaP cells treated with 2′-HF had a significantly lower proliferation level than cells in the control group (P=0.010), yielding a sensitization enhancement ratio of 1.19. The results of TBHP oxidative stress assay suggested that VCaP cells treated with 2′-HF had significantly weaker anti-oxidative capacity than cells in the control group (P=0.015). Hoechst staining and apoptosis flow cytometry with Annexin V-FITC and PI indicated that 2′-HF treatment plus irradiation significantly enhanced apoptosis in VCaP cells (P=0.001. The results of Western blot suggested that 2′-HF treatment significantly inhibited the protein expression of p-AKT and AKR1C3 in VCaP cells (P=0.013 and P=0.016). Conclusions 2′-HF can enhance the radiosensitivity of prostate cancer cells, which is probably associated with its inhibitory effects on AKT pathway and AKR1C3 expression in prostate cancer cells.
Wang Wen,Xiong Wei,Li Xiaoying et al. Radiosensitizing effect and mechanism of 2′-hydroxyflavanone in prostate cancer cells [J]. Chinese Journal of Radiation Oncology, 2016, 25(5): 513-518.
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