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10 cGy单次全脑照射引起大鼠认知功能损害的初步实验研究
孙锐, 张力元, 冀胜军, 陈列松, 嵇建峰, 田野
215004 苏州,苏州大学附属第二医院放疗科
An experimental study of 10 cGy whole brain radiation induced cognitive dysfunction
SUN Rui, ZHANG Li-yuan, JI Sheng-jun, CHEN Lie-song, JI Jian-feng, TIAN Ye
Department of Radiation Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China Corresponding author:TIAN Ye, Email:dryetian@hotmail.com
Abstract：Objective To investigate the relationship between 10 cGy whole brain radiation and cognitive dysfunction. Methods Thirty-two male Sprague-Dawley rats at age one month were randomized into irradiation and sham exposed groups. Behavioral and histopathological tests were performed 3 months after irradiation in the order of open field, Morris water maze, passive avoidance, and histopathological test. Comparison between the two groups was conducted using independent samples t-test. Results In the place navigation test of Morris water maze, irradiation group showed significantly longer latency than sham exposed group on day 3 and 4(t=2.91 and 2.65,all P<0.05). In the total latency of the place navigation, irradiation group also presented longer latency comparing with sham exposed group (t=2.63,P<0.05). In the spatial probe test of Morris water maze, the open field test and the passive avoidance test showed no significant difference between the two groups (t=0.92,0.59,0.83,all P>0.05).The histopathological examination had no significant difference either. Conclusion Whole brain radiation of 10 cGy could partly injury the cognitive function of the rat.
SUN Rui,ZHANG Li-yuan,JI Sheng-jun et al. An experimental study of 10 cGy whole brain radiation induced cognitive dysfunction[J]. Chinese Journal of Radiation Oncology, 2012, 21(5): 474-476.
[1] Soussain C, Ricard D, Fike JR, et al. CNS complications of radiotherapy and chemotherapy. Lancet,2009,374:1639-1651. [2] 张力元,孙锐,田野.电离辐射导致大脑认知功能损害的实验研究进展.中华医学杂志,2012,92:63-67. [3] Kim JS, Lee HJ, Kim JC, et al. Transient impairment of hippocampus-dependent learning and memory in relatively low-dose of acute radiation syndrome is associated with inhibition of hippocampal neurogenesis. J Radiat Res,2008,49:517-526. [4] Achanta P, Fuss M, Martinez JL Jr. Ionizing radiation impairs the formation of trace fear memories and reduces hippocampal neurogenesis. Behav Neurosci,2009,123:1036-1045. [5] Lowe X, Bhattacharya S, Marchetti F, et al. Early brain response to low-dose radiation exposure involves molecular networks and pathways associated with cognitive functions, advanced aging, and Alzheimer′s disease. Radiat Res,2009,171:53-65. [6] Tian Y, Shi Z, Yang S, et al. Changes in myelin basic protein and demyelination in the rat brain within 3 months of single 2-, 10-, or 30-Gy whole-brain radiation treatments. J Neurosurg,2008,109:881-888. [7] Raber J, Wong D, Buttini M, et al. Isoform-specific effects of human apolipoprotein E on brain function revealed in ApoE knockout mice:increased susceptibility of females. Proc Natl Acad Sci USA,1998,95:10914-10919. [8] Choleris E, Thomas AW, Kavaliers M, et al. A detailed ethological analysis of the mouse open field test:effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neurosci Biobehav Rev,2001,25:235-260. [9] Vorhees CV, Williams MT. Morris water maze:procedures for assessing spatial and related forms of learning and memory. Nat Protoc,2006,1:848-858. [10] Raber J, Rola R, LeFevour A, et al. Radiation-induced cognitive impairments are associated with changes in indicators of hippocampal neurogenesis. Radiat Res,2004,162:39-47. [11] Albert MS. Changes in cognition. Neurobiol Aging,2011,32 Suppl 1:58-63. [12] Kitamura T, Saitoh Y, Takashima N, et al. Adult neurogenesis modulates the hippocampus-dependent period of associative fear memory. Cell,2009,139:814-827. [13] Gould E, Tanapat P. Stress and hippocampal neurogenesis. Biol Psychiatry,1999,46:1472-1479. [14] Rola R, Raber J, Rizk A, et al. Radiation-induced impairment of hippocampal neurogenesis is associated with cognitive deficits in young mice. Exp Neurol,2004,188:316-330. [15] Tassoni G, Lorenzini CA, Baldi E, et al. A peculiar pattern of temporal involvement of rat perirhinal cortex in memory processing. Behav Neurosci,1999,113:1161-1169. [16] Iwata Y, Suzuki K, Wakuda T, et al. Irradiation in adulthood as a new model of schizophrenia. PLoS One,2008,3:e2283.
2012, Vol. 21 
