Abstract：Objective To study the dose and time effects of X-ray radiation on the expression of Pokemon gene and protein in human lung adenocarcinoma cell line A549. Methods A549 cells was exposed to different doses of X-ray (2,4,6 and 8 Gy), and the expression of Pokemon mRNA and protein of the cells was detected by using Quantitative real-time PCR and western-blotting at 2, 4, 8, 12, 24, and 48 h after irradiation. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to detect the proliferation of A549 cells at 1,2,3,4,and 5 d after 2 Gy X-ray irradiation. The mock treated A549 cells were used as the control. Results The expression of Pokemon mRNA trended to decrease after irradiated with 4,6 and 8 Gy in the earlier period and increased in the later period with statistical difference at the most time points (t=3.40－154.76,P=0.000－0.041). The expression of Pokemon protein trended to increase and reached the peak at 8 h after irradiated of 2,4,6 and 8 Gy with statistical difference at the most time points (t=4.18－89.64, P=0.000－0.039). Compared with the control, the proliferation of A549 cells was significantly inhibited during 3 to 5 d after irradiation of 2 Gy (t=2.34－18.19, P=0.000－0.040). Conclusions X-ray irradiation may increase the expression of Pokemon mRNA and protein in A549 cells, which might be correlated with radiation-resistance of A549 cells.
LIANG Xiao-fang*,ZOU Yue,JIANG Qi-sheng et al. Effects of X-ray irradiation on the expression of Pokemon gene in human lung adenocarcinoma cell line[J]. Chinese Journal of Radiation Oncology, 2012, 21(1): 83-86.
[1] Maeda T, Hobbs RM, Pandolfi PP. The transcription factor Pokemon:a new key player in cancer pathogenesis. Cancer Res,2005,65:8575-8578. [2] Maeda T, Hobbs RM, Merghoub T, et al. Role of the proto-oncogene Pokemon in cellular transformation and ARF repression. Nature,2005,433:278-285. [3] Pessler F, Pendergrast PS, Hernandez N. Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts. Mol Cell Biol,1997,17:3786-3798. [4] Morrison DJ, Pendergrast PS, Stavropoulos P, et al. FBI-1, a factor that binds to the HIV-1 inducer of short transcripts (IST), is a POZ domain protein. Nucleic Acids Res,1999,27:1251-1262. [5] 王峰,许锡元. p53基因增加肿瘤放射治疗敏感性的机制. 国际肿瘤学杂志,2007,34:342-344. [6] Nijhof JG, Mulder AM, Speksnijder EN,et al. Growth stimulation of UV-induced DNA damage retaining epidermal basal cells gives rise to clusters of p53 overexpressing cells. DNA Repair,2007,6:1642-1650. [7] Shiheido H, Takashima H, Doi N, et al. mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction. PLOS ONE,2011,6:e17898. [8] 傅海青, 罗灿. 电离辐射对小鼠胸腺和脾细胞MDM2mRNA和蛋白表达的影响. 白求恩医科大学学报,2000,26:226-228. [9] Zhao ZH, Wang SF, Yu L, et al. Expression of transcription factor Pokemon in non-small cell lung cancer and its clinical significance. Chin Med J,2008,121:445-449. [10] Aggarwal A, Hunter WJ Ⅲ, Aggarwal H, et al. Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers. Exp Mol Pathol,2010,89:140-148. [11] Laudes M, Christodoulides C, Sewter C, et al. Role of the POZ zinc finger transcription factor FBI-1 in human and murine adipogenesis. J Biol Chem,2004,279:11711-11718. [12] Pessler F, Hernandez N. Flexible DNA binding of the BTB/POZ-domain protein FBI-1. J Biol Chem,2003,278:29327-293235. [13] 李剑峰. Pokemon基因研究进展. 实用肿瘤学杂志,2007,21:98-100. [14] Gelis C, Mavon A, Vicendo P. The contribution of calpains in the down-regulation of Mdm2 and p53 proteolysis in reconstructed human epidermis in response to solar irradiation. Photochem Photobiol,2005,81:975-982. [15] Kamijo T, Bodner S, van de Kamp E, et al. Tumor spectrum in ARF-deficient mice. Cancer Res,1999,59:2217-2222.
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