The role of lncRNA H19 in prognostic evaluation and regulating the radioresistance in colon cancer
Li Linying1,2, Wang Jiangtao3, Cheng Wei1,2, Li Qiang1,2, Jin Xiaodong1,2
1Institute of Modern Physics, Chinese Academy of Sciences/Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences/Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China; 2University of Chinese Academy of Sciences, Beijing 100049, China; 3The First School of Clinical Medicine of Lanzhou University, Lanzhou 730000, China
Abstract：Objective To investigate the role of lncRNA H19 in evaluating prognosis and regulating radioresistance of colon cancer, aiming to provide a new potential target for the diagnosis and treatment of colon cancer. Methods The value of lncRNA H19 in the clinicopathological parameters and prognosis of colon cancer was assessed based on bioinformatics technology. The expression of lncRNA H19 in HCT116 and SW620 cells was regulated through siRNA and overexpression plasmid transfection, respectively. The effect of regulating lncRNA H19 expression on the proliferation, DNA synthesis, radiosensitivity and cell cycle of colon cancer cells after X‐ray irradiation were detected by CCK8, EdU, cell clonogenic survival assay and flow cytometry. Results The expression of lncRNA H19 was significantly upregulated in colon cancer tissues and correlated with poor prognosis in colon cancer patients. LncRNA H19, as a high‐risk gene for colon cancer, had a significant advantage for prognostic assessment of colon cancer (AUC=0.816). Furthermore, the expression of lncRNA H19 was upregulated after X‐ray irradiation in colon cancer cells. Knockdown of lncRNA H19 (siRNA‐H19) significantly increased the radiosensitivity in HCT116 cells, while overexpression of lncRNA H19 (H19‐OE) enhanced the radioresistance in SW620 cells. Moreover, flow cytometry revealed that the G2/M phase arrest induced by X‐ray irradiation was obviously aggravated after siRNA‐H19 treatment in colon cancer cells, which suggested that lncRNA H19 might regulate the radiosensitivity by inhibiting cell cycle progression. Conclusion LncRNA H19 plays a key role in the prognostic assessment and regulating the radiosensitivity in colon cancer, which can be used as a potential target for improving radiosensitivity of colon cancer radiotherapy.
Li Linying,Wang Jiangtao,Cheng Wei et al. The role of lncRNA H19 in prognostic evaluation and regulating the radioresistance in colon cancer[J]. Chinese Journal of Radiation Oncology, 2022, 31(12): 1153-1159.
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