Experimental study of miR‐26a regulating CTGF expression in radiation‐induced heart disease
Yan Rui1, Cai Honghong1, Guo Min2, Song Jianbo3, Xu Xianhai1, Zhang Yarong1, Yu Yang1, Li Sijin1
1Department of Nuclear Medicine, First Hospital of Shanxi Medical University, Taiyuan 030001, China; 2Department of Cardiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China; 3Department of Radiotherapy, Shanxi Bethune Hospital (Shanxi Academy of Medical Sciences),Taiyuan 030032, China
Abstract：Objective To investigate the regulatory effect of miR‐26a in radiation‐induced heart disease (RIHD) mice. Methods C57/BL6 mice were used to establish RIHD models. The cardiac function, fibrosis, the expression levels of collagen 1 (COL1) and connective tissue growth factor (CTGF), and miR‐26a were detected in RIHD mice. Whether CTGF was the target gene of miR‐26a was verified by dual luciferase kit. Moreover, cardiac fibroblasts were transfected with miR‐26a up and miR‐26a down lentivirus vectors to construct the miR‐26a overexpression and underexpression cell models. The expression of CTGF, proliferation, and apoptosis of cardiac fibroblasts were detected. Results In the RIHD mice, heart function was decreased, myocardial fibrosis was remodeled, the expression levels of COL1 and CTGF were up‐regulated, and the expression level of miR‐26a was down‐regulated. Dual luciferase reporter assay confirmed that CTGF was the target gene regulated by miR‐26a. Overexpression of miR‐26a could inhibit the expression of CTGF, suppress the proliferation of cardiac fibroblasts, promote cell apoptosis and secrete collagen. Underexpression of miR‐26a yielded the opposite results. Conclusion MiR‐26a affects the function of cardiac fibroblasts by targeting CTGF and probably mediates the process of radiation‐induced myocardial fibrosis, which may become a new regulatory target of RIHD.
Yan Rui,Cai Honghong,Guo Min et al. Experimental study of miR‐26a regulating CTGF expression in radiation‐induced heart disease[J]. Chinese Journal of Radiation Oncology, 2022, 31(12): 1147-1152.
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