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热疗联合紫杉醇对人舌鳞癌细胞系CAL-27增殖、凋亡和周期影响
周学筱1,2,沈佩1,2,石凡1,2,邵云1,2,丛瑗1,2,徐婷2,,王升志2
1青岛大学口腔医学院,青岛 266003; 2青岛大学附属烟台毓璜顶医院口腔科,烟台 264000
Effect of hyperthermia combined with paclitaxel on proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27
Zhou Xuexiao1,2, Shen Pei1,2, Shi Fan1,2, Shao Yun1,2, Cong Yuan1,2,Xu Ting2, Wang Shengzhi2
1School of Stomatology of Qingdao University, Qingdao 266003, China; 2Department of Stomatology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai 264000, China
Abstract：Objective To observe the effect of hyperthermia combined with paclitaxel on the proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27, and to explore the underlying mechanism. Methods The working concentration of paclitaxel was determined by CCK-8 assay, and the cultured CAL-27 cells were divided into the control, paclitaxel, 42℃ hyperthermia and combined treatment groups. The ability of cell proliferation was detected by colony formation assay, and the cell cycle and apoptosis were determined by flow cytometry. The expression levels of AKT, p-AKT, Bcl-2 and Bax proteins in each group were measured by Western blot. Results Compared with the control group, the proliferation was significantly inhibited and the apoptosis of CAL-27 cells was significantly promoted in the combined treatment, hyperthermia and paclitaxel groups (all P<0.05), and the anti-proliferation and apoptosis-promoting effect in the combined treatment group was significantly better than those in the hyperthermia and paclitaxel groups (all P<0.05). Western blot showed that hyperthermia combined with paclitaxel could significantly up-regulate the expression level of Bax protein and significantly down-regulate the expression levels of P-AKT and Bcl-2 in CAL-27 cells (all P<0.05). Conclusions Hyperthermia combined with paclitaxel can play a synergistic role in inhibiting proliferation and promoting apoptosis of tongue squamous cell carcinoma CAL-27 cells. The mechanism may be related to the inhibition of AKT activation and the activation of Bax/Bcl-2 apoptosis signaling pathway.
. Effect of hyperthermia combined with paclitaxel on proliferation, apoptosis and cycle of human tongue squamous cell carcinoma cell line CAL-27[J]. Chinese Journal of Radiation Oncology, 2022, 31(2): 192-195.
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