Effect and possible mechanism of Salvianolic acid B on radiosensitivity of non-small cell lung cancer
Wang Guohui1, Yang Xiongtao2, Yang Gaoshan3,4
1Department of Radiotherapy, Second Hospital of Hebei Medical University, Shijiazhuang 050051, China; 2Peking University China-Japan Friendship School of Clinical Medicine, National Clinical Research Center for Respiratory Diseases, Beijing 100029, China; 3Department of Biochemistry and Biology College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, China; 4Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease, Shijiazhuang 050091, China
Abstract：Objective To evaluate the effect of Salvianolic acid B on the radiosensitivity of human non-small cell lung cancer cells and investigate its possible mechanism. Methods Non-small cell lung cancer cells A549 and H1299 were cultured in vitro. The toxicity of Salvianolic acid B on non-small cell lung cancer cells was detected by MTT assay. The effect of Salvianolic acid B on the radiosensitivity was assessed by clone formation assay. Transwell chamber assay was used to evaluate the effect of Salvianolic acid B on the migration of tumor cells. Western blot was employed to detect the expression levels of OTUD7B, MMP-2, MMP-9, E-cadherin, Akt and p-Akt regulated by Salvianolic acid B. Results Salvianolic acid B (5 μmol/L) could inhibit the proliferation of A549 and H1299 cells. Clone formation assay showed that Salvianolic acid B increased the radiosensitivity of A549 and H1299 cells, with a radiosensitization ratio of 1.45 and 1.38, respectively. Transwell chamber assay indicated that the ability of cell migration was significantly inhibited by Salvianolic acid B (P<0.05). Western blot revealed that the expression levels of OTUD7B in A549 and H1299 cells were induced by irradiation in a time-dependent manner. Salvianolic acid B could down-regulate the expression levels of MMP-2, MMP-9 and p-Akt, whereas up-regulate the expression level of E-cadherin by down-regulating the expression level of OTUD7B. Conclusions Salvianolic acid B can enhance the radiosensitivity of A549 and H1299 cells. The possible mechanism is that Salvianolic acid B down-regulates the expression level of OTUD7B induced by irradiation and inhibits the epithelial-mesenchymal transition process of tumor cells.
Wang Guohui,Yang Xiongtao,Yang Gaoshan. Effect and possible mechanism of Salvianolic acid B on radiosensitivity of non-small cell lung cancer[J]. Chinese Journal of Radiation Oncology, 2021, 30(12): 1304-1308.
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