miR-205-5p/E2F1signal axis is involved in the regulation of radiosensitivity of glioma cells through suppressing the classical Wnt/β-catenin signaling pathway
Zhou Youdong1, Luo Ran1, Liu Yanting1,Dong Yuanxun1, Ma Jinyang1, Hu Huojun1, Wang Xuguang1, Guo Jinman1, Huang Song1, Yuan Gao1, Fu Changtao1, Wang Lei1, Gao Yan2
1Department of Neurosurgery, Yichang Central People's Hospital, First Clinical Medical College of China Three Gorges University, Yichang 443000, China; 2Department of Oncology, Yichang Central People's Hospital, First Clinical Medical College of China Three Gorges University, Yichang 443000, China
Abstract：Objective To explore the mechanism of miR-205-5p/E2F1signal axis in regulating the glioma U251, U87 radiotherapy resistance. Methods X-ray gradual ascending and intermittent induction method was used to irradiate the glioma U251 cells to establish U251/TR, U87/TR radiation-resistant cell lines. Then, the morphology, migration, invasion and proliferation abilities of cells (U251/TR, U87/TR radiation-resistant cells and U251, U87 radiation-sensitive cells) were analyzed. Luciferase gene detection system and point mutation technique were employed to analyze the mechanism of miR-205-5p and E2F1 gene activity on U251 and U87 radiation-resistant cell lines. Results Compared with the radiation-sensitive U251 cells, the radiation-resistant cells U251/TR, U87/TR showed increased proliferation activity, enhanced migration and invasion abilities and decreased apoptosis under X-ray irradiation. miR-205-5p mimics transfection could down-regulate the expression of E2F1 factor in U251/TR cells, inhibit cell proliferation, invasion and migration and increase the radiosensitivity of U251/TR cells. miR-205-5p mimics transfection combined with with E2F1down-regulation exerted anti-tumor effect and decreased cell tolerance by suppressing the Wnt/β-catenin signaling pathway activity. Conclusions The glioma radiation-resistant cell line U251/TR, U87/TR can be established by X-ray gradual ascending and intermittent induction method. The miR-205-5p/E2F1signal axis exerts tumor-suppressing effect through the classical Wnt/β-catenin signaling pathway, which can be used as an therapeutic target to increase the radiosensitivity of glioma.
Zhou Youdong,Luo Ran,Liu Yanting et al. miR-205-5p/E2F1signal axis is involved in the regulation of radiosensitivity of glioma cells through suppressing the classical Wnt/β-catenin signaling pathway[J]. Chinese Journal of Radiation Oncology, 2021, 30(11): 1188-1194.
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2021, Vol. 30 
