Effect of resveratrol combined with radiotherapy on cervical cancer Hela cell growth and migration by regulating PI3K/Akt/mTOR signaling pathway
Zhang Jianfu1, Zhang Yunjun2, Jiang Bengui3, Mei Haibing4, Zhong Huizhen5
1Department of Pharmacy, Ningbo Women's and Children's Hospital, Ningbo 315012, China; 2Department of Imaging, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; 3Department of Gynecology, Ningbo Women's and Children's Hospital, Ningbo 315012, China; 4Department of Imaging, Ningbo Women's and Children's Hospital, Ningbo 315012, China; 5School of Medicine, Ningbo University, Ningbo 315211, China
Abstract：Objective To evaluate the effect of resveratrol combined with γ-ray irradiation on the biological behavior of cervical cancer cells, and to explore its possible mechanism. Methods The proliferation of cell populations after different concentrations of resveratrol solution±γ-ray irradiation was detected by CCK-8 assay. Scratch test and Transwell chamber test were used to detect cell migration and invasion. Flow cytometry and Annexin V-FITC/PI double staining were employed to assess cell apoptosis. Western blot was performed to measure the expression levels of PI3K, Akt, p-Akt, mTOR and p-mTOR proteins. Results Compared with the normal control (NC) group, the resveratrol group±γ-ray irradiation could inhibit the proliferation, migration, and invasion and promote cell apoptosis of human cervical cancer Hela cells, and the combined effect was more obvious. Compared with the NC group, resveratrol and γ-ray irradiation could significantly down-regulate the expression levels of Bcl-2, PI3K, p-Akt and p-mTOR proteins, up-regulate the expression level of Bax protein, but did not significantly alter the expression levels of Akt and mTOR proteins in human cervic1255al cancer Hela cells. Conclusions Resveratrol combined with γ-ray irradiation can dramatically inhibit the proliferation, migration, invasion, and promote the apoptosis of cervical cancer Hela cells. The mechanism may be related to the inhibition of the PI3K/Akt/mTOR signaling pathway and down-regulating the expression levels of downstream related proteins.
Zhang Jianfu,Zhang Yunjun,Jiang Bengui et al. Effect of resveratrol combined with radiotherapy on cervical cancer Hela cell growth and migration by regulating PI3K/Akt/mTOR signaling pathway[J]. Chinese Journal of Radiation Oncology, 2021, 30(9): 956-960.
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