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miR-424-5p通过靶向抑制HMGA1表达提高宫颈癌放射敏感性
李艳云1, 孙桂霞1, 王宁1, 吴大鹏2, 蒋会娟2
1河南大学淮河医院妇产科,开封 475000; 2河南大学淮河医院放疗科,开封 475000
miR-424-5p improves radiation sensitivity of cervical cancer by targeted inhibition of HMGA1 expression
Li Yanyun1, Sun Guixia1, Wang Ning1, Wu Dapeng2, Jiang Huijuan2
1Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng 475000, China; 2Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, China
Abstract：Objective To investigate the effect of miR-424-5p on radiosensitivity and its mechanism in cervical cancer patients. Methods The expression levels of miR-424-5p in the cervical cancer tissues and Hela cells were detected by RT-qPCR. The apoptosis rate of Hela cells was determined by flow cytometry. The proliferation activity of Hela cells was detected by CCK-8 assay. The protein expression levels in Hela cells were measured by Western blot. Results Compared with normal tissues and cells, the expression level of miR-424-5p was significantly down-regulated in the cervical cancer tissues and Hela cells (1.03 vs. 0.88,P<0.01;1.00 vs. 0.75, P<0.01). Overexpression of miR-424-5p significantly inhibited the proliferation activity of Hela cells after radiation treatment (P<0.01), and significantly increased the apoptosis rate of Hela cells after radiation treatment (24.82% vs. 49.94%,P<0.001). Overexpression of miR-424-5p inhibited HMGA1 expression (1.01 vs. 0.63,P<0.01). miR-424-5p directly affected HMGA1, thereby impacting the radiosensitivity of cervical cancer radiotherapy. Conclusion miR-424-5p can improve the radiosensitivity of cervical cancer radiotherapy by directly targeting HMGA1.
Li Yanyun,Sun Guixia,Wang Ning et al. miR-424-5p improves radiation sensitivity of cervical cancer by targeted inhibition of HMGA1 expression[J]. Chinese Journal of Radiation Oncology, 2021, 30(3): 289-293.
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2021, Vol. 30 
