Effect of long-chain non-coding RNA TUG1 on radiosensitivity of human cervical cancer XB1702 cells by adsorption of miR-145
Liu Xiuling1, Wang Sen2, Wang Zhihong3, Li Jing4, Chen Xinyu5, He Quanqin1
1Department of Gynaecology,Zhumadian Central Hospital,Zhumadian 463000,China; 2Department of Radiotherapy,Zhumadian Central Hospital,Zhumadian 463000,China; 3Department of Obstetrics and Gynecology,Second Affiliated Hospital of Zhengzhou University,450000 Zhengzhou,China; 4Department of Obstetrics and Gynecology,Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China; 5Department of Oncology,Zhumadian Center Hospital,Zhumadian 463000,China
Abstract：Objective To evaluate the effect of long-chain non-coding RNA TUG1 on the radiosensitivity of cervical cancer cells and explore its underlying mechanism. Methods The expression of TUG1 and miR-145 in cervical cancer cells XB1702 and normal endometrial stromal cells (ESCs) was detected by qRT-PCR. The transfected si-NC,transfected si-TUG1,transfected si-NC combined with irradiation,transfected si-TUG1 combined with irradiation,si-TUG1 and anti-miR-NC co-transfected and,si-TUG1 and anti-miR-145 co-transfected groups were established, which were transfected into XB1702 cells by liposome method. The survival fraction of each group was detected by colony formation assay. The cell apoptosis of each group was detected by flow cytometry. The fluorescence activity of each group was assessed by dual luciferase reporter gene assay. Results Compared with the normal ESCs,the expression of TUG1 was significantly up-regulated, whereas that of miR-145 was significantly down-regulated in the cervical cancer cells XB1702. Silencing TUG1 significantly increased the survival fraction of XB1702 cells,promoted cell apoptosis and enhanced the radiosensitivity of irradiation to XB1702 cells. TUG1 could target and regulate the expression of miR-145. Suppressing miR-145 reversed the silencing effect of TUG1 on inhibiting proliferation, accelerating apoptosis promotion and enhancing sensitization of XB1702 cells. Conclusions Silencing long-chain non-coding RNA TUG1 can enhance the radiosensitivity of cervical cancer cells. The mechanism may be related to targeting miR-145,which will provide a target for radiotherapy of cervical cancer.
Liu Xiuling,Wang Sen,Wang Zhihong et al. Effect of long-chain non-coding RNA TUG1 on radiosensitivity of human cervical cancer XB1702 cells by adsorption of miR-145[J]. Chinese Journal of Radiation Oncology, 2019, 28(12): 939-941.
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2019, Vol. 28 
