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STC1基因对A549细胞放射敏感性影响
安安1, 侯良学2, 祁峰1, 李桂英2, 康盛伟2
1河南商丘市第一人民医院放射治疗部 476100; 2河南商丘市第一人民医院放疗科 476100
The effect of STC1 gene on radiosensitivity of human lung cancer A549 cell line
An An1, Hou Liangxue2, Qi Feng1, Li Guiying2, Kang Shengwei2
1Department of Radiotherapy,Shangqiu First People′s Hospital,Shangqiu 476100,China; 2Department of Radiotherapy,Shangqiu First People′s Hospital,Shangqiu 476100,China
Abstract：Objective To investigate effect of stanniocalcin-1(STC1) gene on the proliferation,apoptosis and radiotherapy sensitivity of non-small cell lung cancer.Methods The STC1 siRNA (STC1-siRNA) and the non-interfering siRNA (negative control group) were transfected into the human lung cancer A549 cells by LipofectamineTM2000,and the blank control group was established. The expression level of STC1 protein was detected after transfection for 48 h by Western blotting. Clone forming test was adopted to detect the proliferation of A549 cells after STC1-siRNA and irradiation treatment. CCK8 assay was performed to detect the cell viability after treatment with STC1-siRNA and STC1-siRNA+8 Gy. The cell apoptosis was detected by flow cytometry. The expression levels of Ki67,Bax,STAT3 and p-STAT3 proteins were quantitatively measured by Western blotting.Results The expression level of STC1 protein in the A549 cells transfected with STC1-siRNA was significantly down-regulated than that in the blank control group (P<0.05).Compared with the blank control group,the sensitization ratio was significantly enhanced after STC1-siRNA transfection. Compared with the blank control group,the cell viability and the expression levels of Ki67 and p-STAT3 protein were significantly decreased,whereas the apoptosis rate and the expression of Bax protein were significantly increased in the STC1-siRNA group. Compared with the STC1-siRNA group,the cell viability and the expression levels of Ki67 and p-STAT3 proteins were significantly decreased,whereas the cell apoptosis rate and the expression of Bax protein were remarkably increased in the STC1-siRNA+8 Gy group (all P<0.05). Conclusion Inhibition of STC1 gene expression can enhance the radiotherapy sensitivity and down-regulate the STAT3 signaling pathway in non-small cell lung cancer.
An An,Hou Liangxue,Qi Feng et al. The effect of STC1 gene on radiosensitivity of human lung cancer A549 cell line[J]. Chinese Journal of Radiation Oncology, 2019, 28(6): 445-447.
[1] Hayase S,Sasaki Y,Matsubara T,et al. Expression of stanniocalcin 1 in thyroid sidepopulation cells and thyroid cancercells[J].Thyroid,2015,25(4):425-436.DOI:10.1089/thy.2014.0464. [2] 李妮娅,左雪梅,李莉.斯钙素 1 的表达对肺癌细胞 A549 细胞周期及凋亡的影响[J].中国癌症杂志,2016,26(8):641 -647.DOI:10.19401/j.cnki.1007-3639.2016.08.001. Li NY,Zuo XM,Li L. Effects of calcium 1 expression on cell cycle and apoptosis of A549 in lung cancer cells[J].Chin Cancer J,2016,26(8):641-647.DOI:10.19401/j.cnki.1007-3639.2016.08.001. [3] Shackelford D B,Abt E,Gerken L,et al. LKB1 inactivation dictates therapeutic response of non-small cell lung cancer to the metabolism drug phenformin[J].Cancer Cell,2013,23(2):143-158.DOI:10.1016/j.ccr.2012.12.008. [4] Du Y, Gu, X, Li L,et al,The diagnostic value of circulating stanniocalcin-1 mRNA in non-small cell lung cancer[J].Surg Oncol,104:836-840.DOI:10.1002/jso.21948. [5] Dai D,Wang Q,Li X,et al. Klotho inhibits human follicular thyroid cancer cell growth and promotes apoptosis through regulation of the expression of stanniocalcin-1[J].Oncol Rep,2016,35(1):552-558.DOI:10.3892/or.2015.4358. [6] Warth A,Cortis J,Soltermann A,et al. Tumour cell proliferation (Ki-67) in non-small cell lung cancer:a critical reappraisal of its prognostic role[J].Br J Cancer,2014,111(6):1222-1229.DOI:10.1038/bjc.2014.402. [7] Zhang X,Bi L,Ye Y,et al. Formononetin induces apoptosis in PC-3 prostate cancer cells through enhancing the Bax/Bcl-2 ratios and regulating the p38/Akt pathway[J].Nutr Cancer,2014,66(4):656-661.DOI:10.1080/01635581.2014.894098. [8] Du YZ,Gu XH,Cheng SF,et al. The oncogenetic role of stanniocalcin 1 in lung adenocarcinoma:a promising serum candidate biomarker for tracking lung adenocarcinoma progression[J].Tumor Biol,2016,37(4):5633-5644.DOI:10.1007/s13277-015-4431-x. [9] Xu YH,Lu S.A meta-analysis of STAT3 and phospho-STAT3 expression and survival of patients with non-small-cell lung cancer[J].Eur J Surg Oncol,2014,40(3):311-317.DOI:10.1016/j.ejso.2013.11.012.
2019, Vol. 28 
