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沉默lncRNA UCA1通过上调miR-873-5p表达对胶质瘤细胞放射敏感性影响
袁金金1, 刘宗文1, 宋锐1, 刘俊启2, 樊锐太2
1郑州大学第二附属医院肿瘤放疗科 450014; 2郑州大学第一附属医院肿瘤放疗科 450003
Silencing lncRNA UCA1 affects radiosensitivity of glioma cells by up-regulating miR-873-5p expression
Yuan Jinjin1, Liu Zongwen1, Song Rui1, Liu Junqi2, Fan Ruitai2
1Department of Radiation Oncology, Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China; 2Department of Radiation Oncology, Frist Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, China
Abstract：Objective To investigate the effect of lncRNA UCA1 on the radiosensitivity of in vitro cultured glioma cell lines SHG-44, U87 and U251 by regulating the miR-873-5p expression. Methods The survival of glioma cells SHG-44, U87 and U251 treated with different radiation intensities (0, 2, 4, 6 and 8Gy) was detected by colony formation assay. The expression levels of UCA1 in glioma cells SHG-44, U87 and U251 were measured by qRT-PCR. The radiation-resistant glioma cells U87 and U251 were selected for subsequent study. After silencing UCA1 expression and/or over-expressing miR-873-5p, the cell survival rate was detected by colony formation assay, and the cell apoptosis rate was determined by flow cytometry. The dual luciferase reporter gene assay and qRT-PCR were employed to verify the targeting relationship between UCA1 and miR-873-5p. Results UCA1 was up-regulated in the radiation-resistant U87 and U251 cells. Silencing UCA1 or over-expressing miR-873-5p inhibited the survival of U87 and U251 cells, and promoted the cell apoptosis induced by radiation exposure. miR-873-5p was a target gene of UCA1, and UCA1 negatively regulated the expression of miR-873-5p. The inhibition of miR-873-5p could reverse the effect of silencing UCA1 on the radiosensitivity of glioma cells. Silencing UCA1 increased the inhibitory effect of radiation on the glioma cell U251 xenografts. Conclusion Silencing UCA1 inhibits the survival of glioma cells and promotes the cell apoptosis by up-regulating the expression of miR-873-5p, thereby increasing the radiosensitivity of glioma cells.
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2021, Vol. 30 
