Establishment of an accurate C57BL6/J mouse model of acute radiation-induced enteritis based on small animal radiation research platform
Tu Yeqiang, Tang Qiu, Zhang Peng, Mu Hanzhou, Ni Juan, Zhou Qiong, Yan Dingding, Lyu Xiaojuan
Institute of Cancer Research and Basic Medical Science of Chinese Academy of Sciences,Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital/Key Laboratory of Radiation Oncology, Hangzhou 310022, China
Abstract：Objective To establish an accurate C57BL6/J mouse model of acute radiation-induced enteritis based on small animal radiation research platform (SARRP). Methods Forty-eight female mice were randomly divided into the following four groups:blank control group, 6Gy irradiation group, 9Gy irradiation group and 12Gy irradiation group. Based on the SARRP, the mice in the irradiation groups were exposed to a single fraction dose of 6Gy, 9Gy and 12Gy at a dose rate of 4Gy/min, respectively. The general condition, body weight andpathological changes of the small intestine of mice were observed. ResultsAfter CT scanning, the target area and normal tissues were delineated. According to the dose distribution of the target area and the protection of spinal cord, the AP-PA field irradiation scheme at the isocentric level was adopted. The average irradiation time in the 6, 9 and 12Gy groups was 163, 252 and 328 seconds, respectively.The survival rates of mice in the 6, 9 and 12 Gy groups were 100%, 100% and 50% 15 days after irradiation.The body weight of mice in the 6Gy (P=0.035), 9Gy (P=0.002) and 12Gy groups (P<0.001) was decreased significantly on the 5th day after irradiation, and gradually increased on the 10th day. With the increase of irradiation dose, the villus and gland injury was aggravated. Compared with the blank control group, the villus length in the 9 and 12Gy groups was significantly shorter (both P<0.001), and theintestinal wall thickness in the irradiation groups was significantly thinner (all P<0.001). Conclusions ARRP can provide accurate target location, planned screening and accurate dose delivery in the establishment of C57BL6/J mouse model of acute radiation-induced enteritis. The C57BL6/J mouse model of acute radiation-induced enteritis can be successfully established by a single fraction total-abdominal irradiation of 6-9Gy.
Tu Yeqiang,Tang Qiu,Zhang Peng et al. Establishment of an accurate C57BL6/J mouse model of acute radiation-induced enteritis based on small animal radiation research platform[J]. Chinese Journal of Radiation Oncology, 2020, 29(7): 569-573.
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