Study of the relationship between radiation-induced taste cell injury and radiation mode in mice
Bu Shanshan1, Xu Gang1, Wang Zhonghou2, Liu Hongxiang2, Wang Xiushen1
1Department of Radiation Oncology,Cancer Hospital Affiliated of Zhengzhou University, Zhengzhou 450008,China; 2Regenerative Bioscience Center, Department is Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Georgia 30602, USA
Abstract：Objective To investigate the relationship between radiation mode and radiation-induced taste cell injury in mouse models. Methods The head and neck of adult C57/bl mice were exposed to 8 Gy irradiation for 1, 2 and 3 times and sacrificed at 2, 4, 7 and 14 d after irradiation. Frozen sections of taste papilla tissues were treated with specific markers by immunohistochemical staining. The changes of proliferative cells and the number of type Ⅱtaste cells at different time points under different radiation modes were observed. The effect of different radiation dose patterns upon the taste cells was assessed. Results The count of proliferative cells was decreased significantly on the 2nd day after radiation, and rapidly recovered to normal level on the 4th day after radiation, which was not correlated with the dose mode. The number of type Ⅱ taste cells was decreased on the 2nd day of the first 8 Gy radiation, decreased to the lowest on the 4th day of the second and third 8 Gy radiation, and rose slowly on the 7th day. Conclusions Radiation can initially decrease and subsequently increase the number of proliferative cells to normal range. Besides, it can gradually repair the type Ⅱ taste cell injury. After high-dose irradiation, the decrease of progenitor cells with proliferative function leads to a synchronous decrease in the number of taste function cells, which may be the reason why taste dysfunction cannot be recovered for a long time after radiotherapy.
Bu Shanshan,Xu Gang,Wang Zhonghou et al. Study of the relationship between radiation-induced taste cell injury and radiation mode in mice[J]. Chinese Journal of Radiation Oncology, 2020, 29(10): 901-903.
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2020, Vol. 29 
