Observation of gastric injury induced by single large dose electron beam irradiation in Sprague-Dawly rats
Jin Hualong, Pang Lijuan, Hu Jing, Wu Xiangwei, Yuan Shuiping, Li Sen, Zhan Guangxi, Peng Shumin, Ning Bo
Department of Radiation Oncology (Jin HL,Hu J,Yuan SP,Li S,Ning B),Department of Pathology (Pang LJ),Department of Hepatobiliary Surgery (Wu XW),Department of Obstetrics and Gynecology (Zhan GX) Peng SM,First Affiliated Hospital of the Medical College,Shihezi University,Shihezi 832008,China
Abstract：Objective To observe the gastric changes in adult male Sprague-Dawly (SD) rats irradiated by the single large dose electron beam,providing animal experimental evidence for intraoperative radiotherapy for gastric cancer. Methods Thirty-eight SD rats were randomly divided into the control and experimental groups. The stomach of the rats in the experimental group were subject to single 6 MeV 20 Gy irridiation by using the patent technology of Accurate Irradiation Experiment Table for Small Animal Radiation. The general conditions,gastric injury and body weight change were observed at different days following irradiation. Results The most severe gastric damage of rats was observed on the 14th d after irradiation. The gastric injury was gradually repaired accompanied with glandular atrophy at 28 d post-irradiation,and the gastric injury was manifested as cellulose fibrinous repair on the 56th d after irradiation. Within 1 week post-irradiation,weight loss was noted in the experimental group,which significantly differed from the rats in the control group (P<0.05).During the 2nd week,the body weight was increased in the experimental group,significantly lower compared with the rats in the control group (P<0.05).The body weight of rats did not significantly differ between two groups at 6 weeks after irradiation (P>0.05). Conclusions The most severe gastric injury is observed at 2 weeks after the single-dose 6 MeV electron beam 20 Gy irradiation,whereas no gastric perforation occurs. The gastric injury can be restored to normal status within 8 weeks following irradiation.
Jin Hualong,Pang Lijuan,Hu Jing et al. Observation of gastric injury induced by single large dose electron beam irradiation in Sprague-Dawly rats[J]. Chinese Journal of Radiation Oncology, 2018, 27(10): 937-940.
[1] 中华人民共和国卫生部医政司.胃癌诊疗规范(2011年版)[J].中国医学前沿杂志(电子版),2012,4(5):62-71.DOI:10.3969/j.issn.1674-7372.2012.05.015 Department of Medical Administration,Ministry of Health of the People′s Republic of China. Guidelines for diagnosis and treatment of gastric cancer (2011 Edition)[J].Chin J Front Med Sci (Elect ed),2012,4(5):62-71.DOI:10.3969/j.issn.1674-7372.2012.05.015. [2] 宁博,吴向未.一种小动物放射线精确照射试验台[P].中国专利:ZL 201520967159.0,2016-3-30. Ning B,Wu XW.A small animal radiation accurate irradiation test bench[P].Chinese patent:ZL 201520967159.0,2016-3-30. [3] 杨爱洁,何信佳,安永恒,等.单次大剂量X线照射对大鼠胰腺的损伤作用[J].齐鲁医学杂志,2011,26(4):316-318.DOI:10.3969/j.issn.1008-034(2011)04-0316-03. Yang AJ,He XJ,An YH,et al. Damage of rat pancreas aftera single large-does radiation[J].Med J Qilu,2011,26(4):316-318.DOI:10.3969/j.issn.1008-034(2011)04-0316-03. [4] 邓琳.大鼠急性放射性胃损伤及其保护的研究[D].上海:上海交通大学,2008. Deng L.Study on acute radiation-induced gastric injury and its protection in rats[D].Shanghai:Shanghai Jiao Tong University,2008. [5] 张怀文,胡博,王小平,等.高能X线照射物理参数对皮肤吸收剂量的影响[J].山东医药,2012,52(11):50-53.DOI:10.3969/j.issn.1002-266X.2012.11.017. Zhang HW,Hu B,Wang XP,et al. Influence of physical parameters of high energy X-ray irradiation on skin absorbed dose[J].Shandong Med J,2012,52(11):50-53.DOI:10.3969/j.issn.1002-266X.2012.11.017. [6] 余文军,孟宪文.电子线射野大小对深度剂量分布影响的研究[J].医疗装备,2012,25(9):6-7.DOI:10.3969/j.issn.1002-2376.2012.09.003. Yu WJ,Meng XW.Study on the Influence of electron line field size on depth dose distribution[J].Chin J Med Dev,2012,25(9):6-7.DOI:10.3969/j.issn.1002-2376.2012.09.003. [7] Perez和Brady.放射肿瘤学原理和实践[M]//朱广迎,李晔雄,夏廷毅等译.天津:天津科技翻译出版公司,2012:210,212. Perez and Brady. Principles and Practice of radiation oncology[M]//Zhu GY,Li YX,Xia TY,etc. Tianjin:Tianjin science and technology translation publishing company,2012:210,212. [8] Calvo FA,Meirino RM,Orecchia R.Intraoperative radiation therapy first part:rationale and techniques[J].Crit Rev Oncol Hematol,2006,59(2):106-115.DOI:10.1016/j.critrevonc.2005.11.004. [9] 曾晓清,陈世耀.放射性胃炎诊断和治疗进展[J].现代消化及介入诊疗,2009,14(3):201-203.DOI:10.3969/j.issn.1672-2159.2009.03.025. Zeng XQ,Chen SY.Progress in diagnosis and treatment of radiation gastritis[J].Mod Digest Intervent,2009,14(3):201-203.DOI:10.3969/j.issn.1672-2159.2009.03.025. [10] 宋倩.放射治疗在胃癌治疗中的作用研究[J].中外医疗,2016,35(3):8-10.DOI:10.16662/j.cnki.1674-0742.2016.03.008. Song Q.The role of radiotherapy in the treatment of gastric cancer[J].Chin Fore Med Treat,2014,8(7):99.DOI:10.16662/j.cnki.1674-0742.2016.03.008. [11] 殷蔚伯,余子豪,徐国镇,等.肿瘤放射治疗学[M].4版,北京:中国协和医科大学出版社,2008,94,252-257. Yin WB,Yu ZH,Xu GZ,et al. Tumor radiotherapy[M].V.4.Beijing:China union medical university press,2008,94,252-257. [12] Gomez DR,Tucker S,Martel MK,et al. Predictors of high-grade esophagitis after Three-definitive conformal therapy,intensity-modulated radiation therapy,or proton beam therapy for non-small cell lung cancer[J].Int J Radiat Oncol Biol Phys,2012,84(4):1010-1016.DOI:10.1016/j.ijrobp.2012.01.071. [13] Gazula A,Baldini EH,Chen A,et al. Comparison of once and twice daily radiotherapy for limited stage small-cell lung cancer[J].Lung,2014,192(1):151-158.DOI:10.1007/s00408-013-9518-9. [14] Grover N,Johnson A.Aminocaproic acid used to control upper gastrointestinal bleeding in radiation gastritis[J].Dig Dis Sci,1997,42(5):982-984.DOI:10.1023/A:1018828801379. [15] Singh I,Laungani GB.Intravesical epsilon aminocaproic acid in management of intractable bladder hemorrhage[J].Urology,1992,40(3):227-229.DOI:10.1016/0090-4295(92)90479-G. [16] 郑铭.放射性胃炎[A]//中华医学会消化内镜学分会.2008年全国消化内镜清洗消毒暨消化内镜新进展学术会议暨福建省第六届消化内镜学术会议论文集[C],福州,2008.北京:中华医学会消化内镜学分会,2008:75-76. Zheng M.Radiation gastritis[A],Chinese society of digestive endoscopology.2008 national conference on digestive endoscopy cleaning and digestion endoscopy and the 6th conference of digestive endoscopy in fujian province[C],Fuzhou,2008.Beijing:Chinese society of digestive endoscopology,2008:75-76. [17] 于金明,殷蔚伯,李宝生.肿瘤精确放射治疗学[M].济南:山东科学技术出版社,2004:969-977. Yu JM,Yin WB,Li BS.Tumor precision radiotherapy[M].Ji′nan:Shandong science and technology press,2004:969-977. [18] 华长江,王晓萍.现代术中放疗的进展[J].临床肿瘤学杂志,2012,17(4):375-378.DOI:10.3969/j.issn.1009-0460.2012.04.021. Hua CJ,Wang XP.Progress in modern intraoperative radiotherapy[J].Chin Clin Oncol,2012,17(4):375-378.DOI:10.3969/j.issn.1009-0460.2012.04.021.
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