<sup>125</sup>I粒子植入术对荷神经母细胞瘤裸鼠有效性和安全性实验研究

doi:10.3760/cma.j.issn.1004-4221.2017.05.018

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摘要

目的采用动物实验方法探索 ¹²⁵I粒子植入术治疗神经母细胞瘤的有效性和安全性。方法建立动物模型并分为 ¹²⁵I组、对照组和空白组,每组各15只。每3天测量肿瘤长、短径。每9 d计算肿瘤抑制率,检测肿瘤组织、瘤周组织凋亡、增殖蛋白表达情况,以及裸鼠内分泌指标和骨髓情况。两均数比较采用独立样本t检验。多组间分析采用单因素方差分析。结果肿瘤抑瘤率在第9、18、27天时 ¹²⁵I组均高于对照组、空白组(P值均<0.05),对照组与空白组相近(P>0.05)。肿瘤组织Caspase-3表达 ¹²⁵I组高于对照组、空白组,PCNA表达 ¹²⁵I组低于对照组、空白组(P值均<0.05),对照组和空白组相近(P>0.05)。瘤周组织中Caspase-3、PCNA在各组间表达均相近(P值均>0.05)。肿瘤组织中凋亡细胞数 ¹²⁵I组高于空白组、对照组(P值均<0.05),空白组和对照组相近(P>0.05)。各组间内分泌指标相近(P>0.05)。¹²⁵I组中裸鼠无明显骨髓抑制现象,且与对照组和空白组相近(P值均>0.05)。结论 ¹²⁵I粒子对神经母细胞瘤有明显的杀伤作用,且在治疗剂量内也比较安全。

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	张旺
	杨燕
	吕麟亚
	王珊
	孔祥如
	章均
	杨娇
	王玉蝉

关键词 ：放射性碘粒子, 神经母细胞瘤, 裸鼠, 有效性, 安全性

Abstract：

Objective To examine the efficacy and safety of ¹²⁵I seed implantation for treating neuroblastoma (NB) in animal models.Methods A total of 45 nude mice models of neuroblastoma were constructed and divided into the ¹²⁵I group.control group.and blank group at 15 mice per group. The long and short diameters of the tumor were measured every 3 days.and the tumor inhibition rate was calculated every 9 days. Apoptotic and proliferative protein expression levels in tumor tissue and peritumoral tissue.as well as endocrine markers and bone marrow of the nude mice.were analyzed. The independent sample t test was used to compare the mean scores.and ANOVA was used for comparison between multiple groups. Results Tumor volume inhibition rate was significantly higher in the ¹²⁵I group than in the control group and blank group on days 9.18.and 27(all P<0.05).Caspase-3 expression in tumor tissues was significantly higher in the ¹²⁵I group than in the control group and blank group (all P<0.05).whereas proliferating cell nuclear antigen (PCNA) expression was significantly lower in the ¹²⁵I group than in the control group and blank group (all P<0.05).There was no significant difference in Caspase-3 and PCNA expression between the control group and blank group (all P>0.05).In addition.no significant difference in the expression of Caspase-3 and PCNA in peritumoral tissue was observed between the ¹²⁵I group.control group.and blank group (all P>0.05).Cell apoptosis in tumor tissue was significantly lower in the blank group and control group than in the ¹²⁵I group (all P<0.05).while there was no significant difference between the blank group and the control group (P>0.05).There was no significant difference in endocrine markers between the three groups (P>0.05).There was no significant bone marrow suppression in the ¹²⁵I group.and this observation was similar to those in the control group and blank group (all P>0.05).Conclusions ¹²⁵I seeds have significant toxicity to NB.¹²⁵I seed implantation is safe in nude mice with NB within the therapeutic doses.

Key words： seeds Neuroblastoma Nude mice Efficacy Safety

收稿日期: 2016-06-16

基金资助:

国家临床重点专科建设项目([2013]544)

通讯作者: 吕麟亚,Email:1905469939@qq.com

引用本文:

张旺,杨燕,吕麟亚等. ¹²⁵I粒子植入术对荷神经母细胞瘤裸鼠有效性和安全性实验研究[J]. 中华放射肿瘤学杂志, 2017, 26(5): 565-571.

Zhang Wang,Yang Yan,Lyu Linya et al. Efficacy and safety of ¹²⁵I seed implantation for treating neuroblastoma in nude mice[J]. Chinese Journal of Radiation Oncology, 2017, 26(5): 565-571.

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http://journal12.magtechjournal.com/Jweb_fszlx/CN/10.3760/cma.j.issn.1004-4221.2017.05.018 或 http://journal12.magtechjournal.com/Jweb_fszlx/CN/Y2017/V26/I5/565

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