卡托普利通过抑制CCL-2表达缓解大鼠急性放射性肺损伤的机制研究

doi:10.3760/cma.j.issn.1004-4221.2017.10.021

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摘要目的研究卡托普利对大鼠急性放射性肺损伤的抑制作用及可能机制。方法将64只雌性Wistar大鼠随机分为对照组、照射组和照射+卡托普利低/高剂量组。除对照组外,其余各组均给予右肺单次20 Gy照射建立大鼠急性放射性肺损伤模型。于第1、2、4、8周处死大鼠HE染色观察大鼠肺组织变化,RT-qPCR、蛋白印迹法分别检测肺组织中CCL-2mRNA水平及蛋白含量,免疫组化检测各组大鼠肺组织中巨噬细胞(CD₆₈)数量。采用单因素方差分析。结果卡托普利能够减轻急性放射性肺损伤大鼠肺组织炎症反应(P<0.05),抑制单核细胞在损伤肺组织中聚集(P<0.05),降低CCL-2在大鼠肺组织中的含量(P<0.05)。结论卡托普利可能通过降低CCL-2的表达,从而抑制单核细胞在急性放射性肺损伤大鼠肺组织中的聚集,进而减轻炎症反应。

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	王俊
	卢宏达
	雷章
	吴洪斌
	卢驰

关键词 ：卡托普利, 大鼠, 急性放射性肺损伤, 趋化性细胞因子配体2, 巨噬细胞

Abstract： Objective To explore the inhibitory effect of captopril on acute radiation-induced lung injury in rats and the possible mechanism. Methods Sixty-four female Wistar rats were randomly divided into control group, irradiation group, irradiation+low-dose captopril group, and irradiation+high-dose captopril group. A single dose of 20 Gy was given to the right lung of all rats except those in the control group to establish a rat model of acute radiation-induced lung injury. These rats were sacrificed at 1, 2, 4, and 8 weeks. HE staining was used to observe the pathological changes in lung tissue;RT-PCR and Western blot were used to measure the mRNA and protein expression of CCL-2 in lung tissue;immunohistochemical assay was used to determine the number of monocytes (CD₆₈) in lung tissue. A one-way analysis of variance was performed. Results Captopril significantly reduced lung inflammation in rats with acute radiation-induced lung injury (P<0.05), inhibited the accumulation of monocytes (CD₆₈) in lung tissue (P<0.05), and decreased the content of CCL-2 in lung tissue (P<0.05). Conclusions For rats with acute radiation-induced lung injury, captopril can reduce the expression of CCL-2 to inhibit the accumulation of monocytes in lung tissue and thus attenuate lung inflammation.

Key words： Captopril Rat Acute radiation-induced lung injury Chemoattractant cytokine ligand 2 Macrophage

收稿日期: 2016-08-03

基金资助:国家自然科学基金(81372931、81101550);湖北省自然科学基金(2012FFB05904);武汉市科技攻关计划(2013060602010253)

引用本文:

王俊,卢宏达,雷章等. 卡托普利通过抑制CCL-2表达缓解大鼠急性放射性肺损伤的机制研究[J]. 中华放射肿瘤学杂志, 2017, 26(10): 1209-1213.

Wang Jun,Lu Hongda,Lei Zhang et al. Captopril attenuates lung inflammation through inhibiting the expression of CCL-2 in rats with acute radiation-induced lung injury[J]. Chinese Journal of Radiation Oncology, 2017, 26(10): 1209-1213.

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