|
|
物理·生物·技术
|
最新目录|
下期目录|
过刊浏览|
高级检索
|
[an error occurred while processing this directive]|[an error occurred while processing this directive] |
|
|
| 寡核苷酸Dbait乏氧放射双表达对人宫颈癌细胞的乏氧放射增敏效应 |
| 温林春, 尤传文, 陆锡燕, 辛勇, 章龙珍 |
| 223800 宿迁,南京鼓楼医院集团宿迁市人民医院肿瘤科(温林春、尤传文、陆锡燕)；221002 徐州,徐州医学院附属医院放疗科(辛勇、章龙珍) |
|
|
|
|
摘要 基因-放疗是将放射敏感性调控序列与治疗基因和药物相耦联,在肿瘤放疗同时诱导目的基因和药物在肿瘤内高效表达,从而提高肿瘤放射敏感性,它是近年来肿瘤放疗新策略\[1\]。早期生长反应-1(early growth response-1,Egr-1)启动子在电离辐射诱导下能增强下游基因表达,并可提高放疗效果；乏氧反应元件(hypoxia response elements,HRE)在乏氧条件下可增强下游基因表达水平。笔者利用HRE和Egr-1构建乏氧放射双敏感嵌合性启动子HRE/Egr-1诱导寡核苷酸药物Dbait表达,并观察该重组质粒在乏氧条件下对人宫颈癌Hela细胞的乏氧放射增敏效应。
|
|
|
收稿日期: 2012-03-01
|
| 基金资助:国家自然科学基金(81071831) |
[1] Weichselbaum R,Kufe R,Advani S,et al. Molecular targeting of gene therapy and radiotherapy. Acta Oncol,2001,40: 735-738.
[2] Quanz M,Berthault N,Roulin C,et al. Small-molecule drugs mimicking DNA damage: a new strategy for sensitizing tumors to radiotherapy. Clin Cancer Res,2009,15:1308-1316.
[3] 糜福顺,杨伟志,萤秀玥,等．离体细胞的乏氧照射技术//沈瑜,糜榴顺．肿瘤放射生物学．北京:中国医药科技出版社,2002:57-67．
[4] 文碧秀,王振宇.肿瘤乏氧研究新进展.中华放射肿瘤学杂志,2011,20:524-527.
[5] Quanz M,Chassoux D,Berthault N,et al. Hyperactivation of DNA-PK by double-strand break mimicking molecules disorganizes DNA damage response. PLoS One,2009,4:e6298.
[6] 温林春,章龙珍.新型放射增敏Dbait的研究进展.中华放射肿瘤学杂志,2010,19:142-144.
[7] Greco O,Marples B,Dachs GU,et al. Novel chimeric gene promoters responsive to hypoxia and ionizing radiation. Gene Ther,2002,19:1403-1411. |
|
|
|
2012, Vol. 21 
