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| Study on Tissue Culture of Tetraploid Black Locus Clone and Anatomical Observation on the Development of Adventitious Roots and Structure of Leaves |
| Li Yun,Wang Shuzhi,Tian Yanting,Luo Xiaofang |
| College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, China |
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Abstract A micro-propagation system for tetraploid black locust ( Robinia pseudoacacia ) was established by using its shoots. The optimum medium for shoot multiplication and growth was MS + BA0.5 mg·L-1 + NAA0. 1 mg·L-1 . The best medium for rooting was 1/2 MS + IBA 0.4 mg·L-1 + NAA0.25 mg·L-1. The adventitious root development of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots. It had been proved that survival percentage had been increased by means of the plantlets acclimated through leaf anatomical observation.
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Received: 03 April 2003
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