AbstractObjective To investigate the effect of long non-coding RNA HOX transcript antisense RNA (lncRNA HOTAIR) on the cell proliferation,radiosensitivity and apoptosis of the rectal adenocarcinoma cell lines SW480 and HCT116 in vitro. Methods The expression levels of lncRNA HOTAIR in the rectal adenocarcinoma cell lines (SW480 and HCT116) were assessed by real-time quantitative PCR (RT-qPCR).Silencing of HOTAIR by RNA interference was performed to explore its roles in cell proliferation,radiosensitivity and apoptosis. After treatment with irradiation at a gradient dose,the cell viability was measured and the rate of cell apoptosis was tested. Results Compared with the human rectal epithelial cell lines,the expression of lncRNA HOTAIR was significantly higher in the rectal adenocarcinoma cell lines. The colonic assay demonstrated that the sensitizing enhancement ratios (SERs) were 1.58 and 1.33 for the cells transfected with HOTAIR siRNA in SW480 and HCT116 cell line compared with the control isRNA transfection group. In vitro silencing of lncRNA HOTAIR could enhance the apotosis rate and radiosensitivity of the rectal adenocarcinoma cell line SW480. Conclusion The expression level of lncRNA HOTAIR is correlated with the cellular radiosensitivity,which is probably a parameter for predicting the radiosensitivity of rectal adenocarcinoma cells. Radiotherapy combined with HOTAIR-siRNA can significantly inhibit the cell proliferation,induce cell apoptosis and enhance the radiosensitivity.
Fund:Key Project of Science and Technology Development Plan of Henan Province (112102310121)
Corresponding Authors:
Li Shoumiao,Email:shoumiaoli@126.com
Cite this article:
Li Minghe,Li Shoumiao,Zhang Wei et al. Effect of silencing of long non-coding RNA HOTAIR on radiosensitivity of rectal adenocarcinoma cell lines[J]. Chinese Journal of Radiation Oncology, 2018, 27(12): 1097-1100.
Li Minghe,Li Shoumiao,Zhang Wei et al. Effect of silencing of long non-coding RNA HOTAIR on radiosensitivity of rectal adenocarcinoma cell lines[J]. Chinese Journal of Radiation Oncology, 2018, 27(12): 1097-1100.
[1] 达春丽,王若峥,李瑜,等.食管鳞癌细胞HOTAIR mRNA表达水平与放射敏感性之间关系[J].中华放射肿瘤学杂志,2016,25(2):179-183.DOI:10.3760/cma.j.issn.1004-4221.2016.02.021. Da CL,Wang RZ,Li Y,et al. Relationship between HotAir mRNA expression level and radiosensitivity in esophageal squamous cell carcinoma[J].Chin J Radiat Oncol,2016,25(2):179-183.DOI:10.3760/cma.j.issn.1004-4221.2016.02.021. [2] 魏文金,韩东风,李海林,等.长链非编码RNA HOTAIR对人胶质瘤细胞代谢和生长的影响[J].中华神经外科杂志,2015,31(9):942-947.DOI:10.3760/cma.j.issn.1001-2346.2015.09.021. Wei WJ,Han DF,Li HL,et al. Effects of long-chain non-coding RNA hotair on the metabolism and growth of human glioma cells[J].Chin J Neurosurg,2015,31(9):942-947.DOI:10.3760/cma.j.issn.1001-2346.2015.09.021. [3] Zhou Y,Wang C,Liu X,et al. Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells[J].Oncol Lett,2017,13(3):1143-1148.DOI:10.3892/ol.2017.5587. [4] Yang XD,Xu HT,Xu XH,et al. Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer[J].Oncol Rep,2016,35(1):479-487.DOI:10.3892/or.2015.4397. [5] Wu Y,Xiong Q,Li S,et al. Integrated proteomic and transcriptomic analysis revealslong noncoding RNA HOTAIR promotes hepatocellular carcinoma cell proliferation by regulating opioid growth factor receptor (OGFr)[J].Mol Cell Proteomics.2017, 27.DOI:10.1074/mcp. RA117.000277. [6] 左志贵,于志奇,高显华,等.表皮生长因子受体表达及其下游基因突变与结直肠癌细胞放射敏感性的关系[J].中华胃肠外科杂志,2013,16(8):753-758.DOI:10.3760/cma.j.issn.1671-0274.2013.08.014. Zuo ZG,Yu XQ,Gao XH,et al. Relationship between expression of epidermal growth factor receptor and its downstream gene mutation and radiosensitivity of colorectal cancer cells[J].Chin J Gastrointest Surg,2013,16(8):753-758.DOI:10.3760/cma.j.issn.1671-0274.2013.08.014. [7] 崔广晖,崔明威,李宇航,等.长链非编码RNA HOTAIR对食管鳞癌细胞EC9706恶性生物学行为的影响[J].中华实验外科杂志,2014,31(8):1657-1658.DOI:10.3760/cma.j.issn.1001-9030.2014.08.015. Cui GH,Cui MW,Li YH,et al.长Effects of non-coding RNA hotair on EC9706 malignant biological behavior of esophageal squamous cell carcinoma[J].Chin J Exper Surg,2014,31(8):1657-1658.DOI:10.3760/cma.j.issn.1001-9030.2014.08.015. [8] Wu ZH,Wang XL,Tang HM,et al. Long non-coding RNA HOTAIR is a powerful predictor of metastasis and poor prognosis and is associated with epithelial-mesenchymal transition in colon cancer[J].Oncol Rep,2014,32(1):395-402.DOI:10.3892/or.2014.3186. [9] 李建成,张和平,刘迪,等.表皮生长因子受体siRNA对食管鳞癌细胞的放射增敏研究[J].中华放射医学与防护杂志,2013,33(1):23-26.DOI:10.3760/cma.j.issn.0254-5098.2013.01.006. Li JC,Zhang HP,Liu D,et al. Radiation sensitization of epidermal growth factor receptor siRNA on esophageal squamous cell carcinoma[J].Chin J Radiol Med Protect,2013,33(1):23-26.DOI:10.3760/cma.j.issn.0254-5098.2013.01.006. [10] Lu X,Liu Z,Ning X,et al. The long noncoding RNA HOTAIR promotes colorectal cancer progression by sponging miR-197[J].Oncol Res,2017 Nov 14.DOI:10.3727/096504017X15105708598531. [11] Cheng C,Qin Y,Zhi Q,et al. Knockdown of long non-coding RNA HOTAIR inhibits cisplatin resistance of gastric cancer cells through inhibiting the PI3K/Akt and Wnt/β-catenin signaling pathways by up-regulating miR-34a[J].Int J Biol Macromol,2017 Oct 26.DOI:10.1016/j.ijbiomac.2017.10.154. [12] Hui Z,Xianglin M.Association of HOTAIR expression with PI3K/Akt pathway activation in adenocarcinoma of esophagogastric junction[J].Open Med,2016,11(1):36-40.DOI:10.1515/med-2016-0008. [13] Gupta RA,Shah N,Wang KC,et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J].Nature,2010,464(7291):1071-1076.DOI:10.1038/nature08975. [14] Kogo R,Shimamura T,Mimori K,et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers[J].Cancer Res,2011,71(20):6320-6326.DOI:10.1158/0008-5472. [15] Jing L,Yuan W,Ruofan D,et al. HOTAIR enhanced aggressive biological behaviors and induced radio-resistance via inhibiting p21 in cervical cancer[J].Tumour Biol,2015,36(5):3611-3619.DOI:10.1007/s13277-014-2998-2. [16] Chen J,Shen Z,Zheng Y,et al. Radiotherapy induced Lewis lung cancer cell apoptosis via inactivating β-catenin mediated by upregulated HOTAIR[J].Int J Clin Exp Pathol,2015,8(7):7878-7886. [17] Jiang Y,Li Z,Zheng S,et al. The long non-coding RNA HOTAIR affects the radiosensitivity of pancreatic ductal adenocarcinoma by regulating the expression of Wnt inhibitory factor 1[J].Tumour Biol, 2016,37(3):3957-3967.DOI:10.1007/s13277-015-4234-0. [18] Ertosun MG,Hapil FZ,Osman Nidai O.E2F1 transcription factor and its impact on growth factor and cytokine signaling[J].Cytokine Growth Factor Rev.2016,31(1):17-25.DOI:10.1016/j.cytogfr.2016.02.001. [19] Kong L,Zhou X,Wu Y,et al. Targeting HOTAIR induces mitochondria related apoptosis and inhibits tumor growth in head and neck squamous cell carcinoma in vitro and in vivo[J].Curr Mol Med,2015,15(10):952-960.DOI:10.2174/1566524016666151123112716.
2018, Vol. 27 
