塞来昔布对人脑微血管内皮细胞放射后凋亡及释放PGI<sub>2</sub>和TXA<sub>2</sub>影响

doi:10.3760/cma.j.issn.1004-4221.2017.06.016

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Abstract Objective To investigate the effect of Celecoxib on human brain microvascular endothelial cells release6-keto-PGF_1α,TXB₂ and apotosis after irradiation. Methods The logarithmic growth phase cells were divided into control groups (Con),simple irradiation (IR) groups and combination groups (IR+C).CCK-8 and clone formation experiment were used to evaluate the effects of radiosensitivity and toxicity of celecoxib. The results were observed atthe time point of 6 h,12 h,24 h,48 h after irradiation. ELISA was used to test the contents of 6-keto-PGF_1α and TXB₂,which metabolized by PGI₂ and TXA₂ from culture medium after irradiation at different time points in different groups. TXB2/6-keto-PGF_1αratios were calculated. Annexin V-FITC/PI double staining method was used to measure the apoptosis rates at different time points in different groups. Western blot was used to measure the protein expression. Paired t test difference. Results Compared with simple irradiation group,there were no significant radiosensitivity (SER=0.96) in combination groups incubated with30 μmol/L of celecoxib. Compared with the control group,the ratio of TXB2/6-keto-PGF_1αincreased at each time point in IR and IR+C (P<0.05),and the apoptosis rates increased (P<0.05).Cox-2,P-JNK and Cleaved caspase-3 increased. Compared with IR,the ratio of TXB2/6-keto-PGF_1αdecreased at each time point in IR+C (P<0.05),and the apoptosis rates decreased (t=3.34~6.38,P< 0.05).The protein expression of Cox-2,P-JNK and Cleaved caspase-3 decreased. Conclusions Celecoxib may help to protect HBMECs from releasing TXA₂ and decreasing the ratio of TXB2/6-keto-PGF_1α,and inhibitting apoptosis after irradiation. The mechanisms of apoptosis inhibition may be related to the inhibition of Cox-2 and P-JNK,caspase-3 Cleaved proteinexpressions.

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	Sun Jiaxing
	Xu Xiaoting
	Tu Yu

Key words： Human brain microvascular endothelial cells Cox-2 Celecoxib Irradiation Prostacyclin Thromboxane Apoptosis

Received: 16 November 2016

Corresponding Authors: Xu Xiaoting,Email:szting110@163.com

Cite this article:

Sun Jiaxing,Xu Xiaoting,Tu Yu. The effects of Celecoxib on human brain microvascular endothelial cells release 6-keto-PGF_1α and TXB₂ and apoptosis after radiation[J]. Chinese Journal of Radiation Oncology, 2017, 26(6): 682-686.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2017.06.016 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2017/V26/I6/682

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