Department of Radiation Oncology (Chen BY,Gu HM,Zhou SZ,Yuan JP,Qain X), Department of Gastroenterology (Li XY,Xiao MB,Jiang F),Affiliated Hospital of Nantong University,Nantong 226001,China
AbstractObjective To investigate the effects of high-energy X-ray irradiation on collagen expression in human fetal lung fibroblasts (HFL-1) and identify the genes related to collagen production induced by X-ray, and to provide a theoretical basis for the clinical treatment of radiation-induced pulmonary fibrosis. Methods HFL-1 cells cultured in vitro were randomly divided into control group and irradiation group. The irradiation group was irradiated with 6 MV X-ray at a single dose of 5 Gy. At 24 hours after irradiation, the expression of hydroxyproline (HYP) in the two groups was determined by the digestion method. The expression of collagen types Ⅰ and Ⅲ at mRNA and protein levels was measured by RT-PCR and Western blot. And gene chip was used to identify the differentially expressed genes between the two groups. Results At 24 hours after irradiation, the irradiation group had significantly higher expression of HYP than the control group (1.834 μg/ml vs.4.912 μg/ml, P=0.000), and the irradiation group had significantly higher mRNA and protein expression of collagen types Ⅰ and Ⅲ than the control group (18.535∶vs. 86.779, P=0.000;4.337∶vs. 4.425, P=0.000). A total of 1879 genes were differentially expressed between the two groups, including 771 up-regulated genes and 1108 down-regulated genes in the irradiation group. The genes involved in fibrosis which were up-regulated more than 5-fold were transforming growth factor (TGF)-beta 1, TGF-beta 3, matrix metalloproteinase (MMP)-28, MMP-26, MMP-27, and SMAD6. Conclusions High-energy X-ray can induce collagen production in HFL-1 cells. Gene chip can effectively identify the genes related to collagen production induced by X-ray. Many different genes areinvolved in the development and progression of radiation-induced pulmonary fibrosis.
Qian Xia,Li Xiaoyan,Xiao Mingbing et al. Application of gene chip in identifying genes related to collagen production induced by X-ray in HFL-1 cells[J]. Chinese Journal of Radiation Oncology, 2017, 26(3): 329-334.
Qian Xia,Li Xiaoyan,Xiao Mingbing et al. Application of gene chip in identifying genes related to collagen production induced by X-ray in HFL-1 cells[J]. Chinese Journal of Radiation Oncology, 2017, 26(3): 329-334.
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2017, Vol. 26 
