中华放射肿瘤学杂志
Sunday, Apr. 6, 2025   Home | Journal | Editorial | Instruction | Subscription | Advertisement | Academic | Index-in | Contact Us | Chinese
Chinese Journal of Radiation Oncology  2016, Vol. 25 Issue (6): 628-633    DOI: 10.3760/cma.j.issn.1004-4221.2016.06.019
Physics·Biology·Technology Current Issue| Next Issue| Archive| Adv Search [an error occurred while processing this directive] | [an error occurred while processing this directive]
Models of acute radiation enteritis:a comparison and evaluation of two modeling methods
Wang Yingjie,Wang Shunjin,Gong Lianggeng,Wu Hailong
Department of Oncology (Wang YJ,Wang SHJ),Departmeng of MR (Gong LG,Wu HL),Second Affiliated Hospital of Nanchang University,Nangchang 330006,China
Download: PDF (2787 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract  Objective To investigate the reliable Methods for establishing models of acute radiation enteritis (ARE) and the criteria used to judge whether the model is successfully established. Methods A total of 98 rats were randomly divided into normal control group (group A), fractionated dose group B (4 Gy/fraction for 3 fractions), fractionated dose group C (4 Gy/fraction for 4 fractions), fractionated dose group D (4 Gy/fraction for 5 fractions), single fraction group E (12 Gy in a single fraction), single fraction group F (16 Gy in a single fraction), and single fraction group G (20 Gy in a single fraction). Abdominal irradiation was performed for all rats, and the changes in body weight and defecation were observed. Magnetic resonance imaging (MRI) was performed on days 3-5 after irradiation, and on the 4th day, anatomy was performed to measure the length of small intestine with edema, blood samples were collected to measure endotoxins, and the specimens of small intestine were collected to observe pathological changes. The independent-samples t-test was used for comparison between groups. Results After irradiation, groups D, E, F, and G experienced varying degrees of diarrhea and had positive results from endotoxins test. Group D had a longer length of small intestine with edema than group C (P=0.00) and had a similar length as group E (P=0.46). Groups E, F, and G showed dilation and dropsy in the intestinal canal on MRI, and groups F and G showed patchy signals of dropsy in the abdominal cavity. Groups F and G showed varying degrees of necrosis in the small intestine and died within 14 days after irradiation. Conclusions When the radiation dose is 33-46 Gy (biologically equivalent dose), both single dose and fractionated dose can successfully establish the model of ARE, while fractionated dose can be better controlled.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Wang Yingjie
Wang Shunjin
Gong Lianggeng
Wu Hailong
Key words Animal model      Acute radiation enteritis      Radiation-induced intestinal injury     
Received: 04 May 2015     
Corresponding Authors: Wang Shunjin,Email:wangshunjin1950@163.com   
Cite this article:   
Wang Yingjie,Wang Shunjin,Gong Lianggeng et al. Models of acute radiation enteritis:a comparison and evaluation of two modeling methods[J]. Chinese Journal of Radiation Oncology, 2016, 25(6): 628-633.
Wang Yingjie,Wang Shunjin,Gong Lianggeng et al. Models of acute radiation enteritis:a comparison and evaluation of two modeling methods[J]. Chinese Journal of Radiation Oncology, 2016, 25(6): 628-633.
URL:  
http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2016.06.019     OR     http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2016/V25/I6/628
  Copyright © 2010 Editorial By Chinese Journal of Radiation Oncology
Support by Beijing Magtech Co.ltd  support@magtech.com.cn