2′-羟基黄烷酮对前列腺癌细胞的放射增敏作用及机制探讨

doi:10.3760/cma.j.issn.1004-4221.2016.05.020

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Abstract Objective To study the radiosensitizing effect of 2′-hydroxyflavanone (2′-HF) on prostate cancer cells, and to preliminarily investigate its mechanism. Methods Colony formation assay, tert-butylhydroperoxide (TBHP) oxidative stress assay, Hoechst staining, and apoptosis flow cytometry using Annexin V-FITC and propidium iodide (PI) were performed to measure the impact of 2′-HF on the radiosensitivity of VCaP prostate cancer cells. Western blot was used to determine the effects of 2′-HF on expression of AKT, phosphorylated AKT (p-AKT), and aldo-keto reductase 1C3(AKR1C3) in VCaP cells and preliminarily investigate the mechanism. Data were analyzed by t test and factorial analysis of variance. Results The results of colony formation assay indicated that after exposure to radiation, VCaP cells treated with 2′-HF had a significantly lower proliferation level than cells in the control group (P=0.010), yielding a sensitization enhancement ratio of 1.19. The results of TBHP oxidative stress assay suggested that VCaP cells treated with 2′-HF had significantly weaker anti-oxidative capacity than cells in the control group (P=0.015). Hoechst staining and apoptosis flow cytometry with Annexin V-FITC and PI indicated that 2′-HF treatment plus irradiation significantly enhanced apoptosis in VCaP cells (P=0.001. The results of Western blot suggested that 2′-HF treatment significantly inhibited the protein expression of p-AKT and AKR1C3 in VCaP cells (P=0.013 and P=0.016). Conclusions 2′-HF can enhance the radiosensitivity of prostate cancer cells, which is probably associated with its inhibitory effects on AKT pathway and AKR1C3 expression in prostate cancer cells.

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	Wang Wen
	Xiong Wei
	Li Xiaoying
	Gao Xianshu
	Sun Shaoqian
	Li Yi

Key words： 2′-hydroxyflavanone Radiosensibility Prostate cancer cells line

Received: 01 February 2016

Corresponding Authors: Gao Xianshu,Email:gao7777@139.com:Li Xiaoying,Email:13716109164@139.com

Cite this article:

Wang Wen,Xiong Wei,Li Xiaoying et al. Radiosensitizing effect and mechanism of 2′-hydroxyflavanone in prostate cancer cells　[J]. Chinese Journal of Radiation Oncology, 2016, 25(5): 513-518.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2016.05.020 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2016/V25/I5/513

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