食管鳞癌细胞HOTAIR mRNA表达水平与放射敏感性之间关系

doi:10.3760/cma.j.issn.1004-4221.2016.02.021

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Abstract Objective To investigate the relationship between the expression level of HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma (ESCC). Methods Four ESCC cell lines (K150, K450, TE-1, and Eca109) were used in this study. Quantitative real-time polymerase chain reaction was applied to measure the expression level of HOTAIR in the above cell lines;colony-forming assay was applied to measure the survival fraction of different cells irradiated by different doses of X-ray. The t-test or analysis of variance was applied for analysis of differences. The correlation analysis was used by Pearson methods. Results The four cell lines all showed high expression levels of HOTAIR and radioresistance. Compared with the other three cell lines, Eca109 had a lower expression level of HOTAIR, a lower survival fraction at each radiation dose point, and significantly lower D₀ and D_q. The mRNA expression level of HOTAIR and radiosensitivity were K150<TE-1<K450<Eca109 from low to high. The correlation analysis of the expression level of HOTAIR and D₀/D_q revealed that the expression level of HOTAIR was positively correlated with SF₂ and D₀(r=0.643 and 0.777,respectively,both P<0.05). Conclusions There is a significant correlation between the expression level of HOTAIR and cellular radiosensitivity, and the expression level of HOTAIR may be an indicator to predict cellular radiosensitivity.

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	Articles by authors
	Da Chunli
	Wang Ruozheng
	Li Yu
	Li Yawei
	Liu Kai

Key words： HOTAIR mRNA expression Radiosensitivity Cell Line,Esophageal squamous cell carcinoma
Fund program:Natural Science Fund of Xinjiang Uygur Autonomous Region (2015211C115)

Received: 13 July 2015

Corresponding Authors: Wang RZ, Email:wrz8526@163.com

Cite this article:

Da Chunli,Wang Ruozheng,Li Yu et al. Relationship between the expression of long-non-coding RNA (lncRNA) HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma[J]. Chinese Journal of Radiation Oncology, 2016, 25(2): 179-183.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2016.02.021 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2016/V25/I2/179

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