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Resveratrol up regulates the expression of SIRT1 and mediates protective effect on radiation-induced myocardial injury
Xu Yilin, Chen Yanxin, Cai Yini, Xu Peng, Zeng Zhimin, Huang Long, Liu Anwen
Department of Oncology, the Second Affiliated Hospital of Nanchang University, Jiangxi Key Laboratory of Clinical Translational Cancer Research, Radiation Induced Heart Damage Institute of Nanchang University, Nanchang 330006, China
AbstractObjective To evaluate the effect of resveratrol on radiation-induced myocardial injury in mice. Methods A total of 80 C57BL/6 mice were randomly divided into the control group, resveratrol (Res) group, radiation (RT) group and radiation+resveratrol (RT+Res) group. In the RT group, mice were given with heart radiation and mice in the Res group were given with resveratrol by gavage for 3 months. Cardiac ultrasound was used to evaluate cardiac function at 3 months after cardiac radiation. The hearts of mice were collected for HE staining, immunofluorescence, TUNEL staining, Masson staining and Western blot to evaluate the expression of silent information regulator 1 (SIRT1), the level of oxidative stress, inflammatory response, apoptosis and the degree of fibrosis in myocardial tissues. Experimental data were expressed as Mean ± SD. Continous data were statistically analyzed by t-test. Results After 3 months of irradiation, compared with the control group, the ejection fraction (EF) and fractional shortening (FS) of cardiac function were decreased, and myocardial degeneration and disorder, reactive oxygen species (ROS) and inflammatory levels (interleukin-1β, interleukin-6, tumor necrosis factor-α), myocardial apoptosis (TUNEL positive cell rate) and fibrosis were increased in the RT group. In the RT+Res group, the cardiac function was improved, the expression of SIRT1 was increased, and the levels of oxidative stress, inflammation, myocardial apoptosis and fibrosis were decreased. Conclusions Resveratrol can reduce oxidative stress, inflammatory infiltration, apoptosis and fibrosis of myocardium in mice with radiation-induced myocardial injury, thereby improving cardiac structural abnormalities and cardiac dysfunction. This protective effect can be mediated by upregulation of SIRT1 expression.
Fund:National Nature Science Foundation of China (82060577)
Corresponding Authors:
Liu Anwen, Email: awliu666@163.com
Cite this article:
Xu Yilin,Chen Yanxin,Cai Yini et al. Resveratrol up regulates the expression of SIRT1 and mediates protective effect on radiation-induced myocardial injury[J]. Chinese Journal of Radiation Oncology, 2023, 32(5): 457-463.
Xu Yilin,Chen Yanxin,Cai Yini et al. Resveratrol up regulates the expression of SIRT1 and mediates protective effect on radiation-induced myocardial injury[J]. Chinese Journal of Radiation Oncology, 2023, 32(5): 457-463.
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