lncRNA TTN-AS1调控miR-107/HMGA1信号轴影响乳腺癌细胞放射敏感性的研究

doi:10.3760/cma.j.cn113030-20220808-00266

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Abstract Objective To investigate the effect and mechanism of long non-coding RNA (lncRNA) TTN-AS1 on the radiosensitivity of breast cancer cells. Methods The expression of TTN-AS1 in breast cancer cells was detected by real-time reverse transcription PCR (qRT-PCR). MDA-MB-231 cells were divided into the 0 Gy group, 4 Gy group, negative control (NC) +4 Gy group, si-TTN-AS1+4 Gy group, si-TTN-AS1+miR-107 inhibitor+4 Gy group, and si-TTN-AS1+miR-107 inhibitor+si-HMGA1+4 Gy group. CCK-8 assay and flow cytometry were used to detect the proliferation and apoptosis rates in each group. Results Compared with breast epithelial cells, TTN-AS1 was significantly highly expressed in breast cancer cell lines (P<0.001). Compared with the NC+4 Gy group, the cell proliferation ability was significantly decreased (P<0.05) and cell apoptosis was significantly increased (P<0.001) in the si-TTN-AS1+4 Gy group. Compared with the 0 Gy group, the expression levels of TTN-AS1 and HMGA1 from 8 h to 24 h after radiotherapy were significantly up-regulated (both P<0.01), whereas the expression of miR-107 was significantly down-regulated from 8 h to 24 h after radiotherapy in the 4 Gy group (P<0.001). The cell proliferation ability in the si-TTN-AS1+miR-107 inhibitor+4 Gy group was significantly higher than that in the si-TTN-AS1+4 Gy group (P<0.001), and cell apoptosis was significantly lower than that in the si-TTN-AS1+4 Gy group (P<0.001). Compared with the si-TTN-AS1+miR-107 inhibitor+4 Gy group, cell proliferation ability was significantly decreased (P<0.001), whereas cell apoptosis was significantly increased in the si-TTN-AS1+miR-107 inhibitor+si-HMGA1+4 Gy group (P<0.001). Conclusion TTN-AS1 can promote the radiosensitivity of breast cancer cells by regulating the miR-107/HMGA1 signaling axis.

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	Mao Xinhui
	Wang Zhen
	Zhang Jianqing
	Zhu Chengbin

Key words： Breast neoplasms TTN-AS1 Radiosensitivity

Received: 08 August 2022

Fund:Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01C642)

Corresponding Authors: Zhu Chengbin, Email: 1464381784@qq.com

Cite this article:

Mao Xinhui,Wang Zhen,Zhang Jianqing et al. LncRNA TTN-AS1 affects the radiosensitivity of breast cancer cells by regulating miR-107/HMGA1 signaling axis[J]. Chinese Journal of Radiation Oncology, 2023, 32(5): 451-456.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.cn113030-20220808-00266 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2023/V32/I5/451

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