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The role of miR-21 in Endostar combined with X-ray irradiation of cardiac fibroblasts
Cao Dongdong1,2, Ouyang Weiwei1,2, Zhao Xing3, Su Shengfa1, Zhang Jun4, Fu Shimei1,2, Feng Zhangxin2, Li Qingsong2, Ma Zhu1, Yang Wengang2, Chen Xiaxia2, Lu Bing1,2
1Affiliated Hospital of Guizhou Medical University/Department of Oncology, Affiliated Cancer Hospital of Guizhou Medical University, Guiyang 550004, China; 2Teaching and Research Section of Oncology, Guizhou Medical University, Guiyang 550004, China; 3Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang 550004, China; 4Department of Pathology, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China
AbstractObjective To investigate the implication of micro RNA-21(miR-21) in Endostar combined with X-ray irradiation of cardiac fibroblasts (CF). Methods Rat CFs were used in this experiment and been divided into the blank control group, 10Gy X-ray irradiation group, Endostar group, 10Gy X-ray+Endostar group, 10Gy X-ray+Endostar+NC mimic group (negative control 1), 10Gy X-ray+Endostar+miR-21 mimic group, 10Gy X-ray+Endostar+NC inhibitor group (negative control 2) and 10Gy X-ray+Endostar+miR-21 inhibitor group. The proliferation of CF was determined by Methyl thiazolyl tetrazolium (MTT) assay. The expression level of Collagen Ⅰ protein was analyzed by Western blot. The expression levels of Collagen Ⅰ and miR-21 mRNA were assayed by real-time quantitative polymerase chain reaction (q-PCR). Results In the 10Gy X-ray+Endostar+miR-21 mimic group, the CF proliferation, Collagen Ⅰ and miR-21 mRNA were increased significantly compared with those in the blank control group, 10Gy X-ray+Endostar group, and negative control group 1 (all P<0.05). In the 10Gy X-ray+Endostar+miR-21 inhibitor group, the CF proliferation and expression levels of Collagen Ⅰ mRNA were decreased significantly compared with those in the blank control group, 10Gy X-ray+Endostar group and negative control group 2(all P<0.05). Conclusions The CF proliferation and Collagen Ⅰ expression are increased when the expression level of miR-21 gene is simulated. When inhibiting the expression of miR-21 gene, the CF proliferation and Collagen Ⅰ expression are reduced.
Fund:National Natural Science Foundation of China (NSFC81660507);China International Medical Exchange Foundation (Z-2014-06-19393);Project of the Science and Technology Department of Guizhou Province ([2014]7135);Major Projects of Application of Basic Research Program of Guizhou Province ([2015]2003)
Corresponding Authors:
Ouyang Weiwei, Email: ouyangww103173@163.com; Lu Bing, Email: lbgymaaaa@163.com
Cite this article:
Cao Dongdong,Ouyang Weiwei,Zhao Xing et al. The role of miR-21 in Endostar combined with X-ray irradiation of cardiac fibroblasts[J]. Chinese Journal of Radiation Oncology, 2022, 31(4): 370-375.
Cao Dongdong,Ouyang Weiwei,Zhao Xing et al. The role of miR-21 in Endostar combined with X-ray irradiation of cardiac fibroblasts[J]. Chinese Journal of Radiation Oncology, 2022, 31(4): 370-375.
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