下调lncRNA LINC00263靶向miR-4458调控乳腺癌SK-BR-3细胞放射敏感性

doi:10.3760/cma.j.cn113030-20200803-00393

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Abstract Objective To evaluate the effect of down-regulating lncRNA LINC00263 targeting miR-4458 on the proliferation, migration, invasion and radiosensitivity of breast cancer SK-BR-3 cells. Methods The expression differences of LINC00263 in breast cancer tissues, adjacent tissues, normal breast epithelial cells and breast cancer cells were determined by qRT-PCR. Transfection of LINC00263shRNA in breast cancer SK-BR-3 cells down-regulated the expression of LINC00263, and the cloning experiment was used to detect the radiosensitivity. Breast cancer SK-BR-3 cells were treated with 6Gy irradiation. CCK-8 assay was employed to detect cell proliferation. Flow cytometry was adopted to detect cell apoptosis. Transwell chamber test was performed to detect cell migration and invasion. Western blot was used to detect the expression levels of C-Caspase-3 and C-Caspase-9, MMP-2 and MMP-9 proteins. Bioinformatics software predicted that LINC00263 and miR-4458had complementary binding sites, and the luciferase reporter system was utilized determine the targeting relationship between LINC00263 and miR-4458. LINC00263shRNA and miR-4458 inhibitor were co-transfected into breast cancer SK-BR-3 cells, and 6Gy irradiation was given to detect the changes in cell proliferation, apoptosis, invasion and migration. Results The expression level of LINC00263 in breast cancer tissues was higher than that in adjacent tissues. The expression level of LINC00263 in breast cancer cells was higher compared with that in normal breast epithelial cells. The radiosensitivity of breast cancer SK-BR-3 cells was increased after transfection of LINC00263shRNA. Transfection of LINC00263shRNA and radiation exerted a synergistic effect, jointly inhibited breast cancer cell proliferation, migration and invasion, promoted cell apoptosis, up-regulated the expression levels of C-Caspase-3 and C-Caspase-9 proteins in cells, and down-regulated those of MMP-2 and MMP-9 proteins. Down-regulation of LINC00263 targetedly up-regulated miR-4458 expression. miR-4458 inhibitor reversed the inhibitory effect of LINC00263shRNA combined with radiation on the proliferation, migration, invasion and apoptosis promotion of breast cancer SK-BR-3 cells. Conclusion Down-regulating lncRNA LINC00263 targeting miR-4458 inhibits the proliferation, migration and invasion of breast cancer SK-BR-3 cells, and improves cell radiosensitivity.

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	Wen Lanlan
	Wang Dongjuan
	Dong Hui
	Zhao Jiwei
	Zhu Cuimin
	Lin Pingping
	Liu Lanfang
	Li Qingshan

Key words： LINC00263 gene miR-4458 gene Radiosensitivity SK-BR-3 cells line

Received: 03 August 2020

Corresponding Authors: Li Qingshan, Email:tuanrana5141@126.com

Cite this article:

Wen Lanlan,Wang Dongjuan,Dong Hui et al. Effect of down-regulation of lncRNA LINC00263 targeting miR-4458 on regulating radiosensitivity of breast cancer SK-BR-3 cells[J]. Chinese Journal of Radiation Oncology, 2021, 30(11): 1195-1201.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.cn113030-20200803-00393 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2021/V30/I11/1195

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