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LncRAN MEG3 regulates the radiosensitivity of cervical cancer cells by targeting miR-181a-5p
Hou Ge1, Wang Cheng1, Li Ruping2, Xiao Chenhu1, Chu Alan1, Liu Zongwen1
1Department of Radiation Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China; 2Department of Nuclear Medicine, Henan Cancer Hospital, Zhengzhou 450003, China
AbstractObjective To evaluate the effect of long-chain non-coding RNA MEG3(LncRNA MEG3) on the radiosensitivity of cervical cancer cells, and to explore its underlying mechanism. Methods The expression of LncRNA MEG3 in cervical cancer cells was detected by qRT-PCR. In the overexpression control group (transfected with pcDNA 3.1), LncRNA MEG3 overexpression group (transfected with pcDNA 3.1-LncRNA MEG3), miR-NC inhibition group (transfected with anti-miR-NC), miR-181a-5p inhibition group (transfected with anti-miR-181a-5p), LncRNA MEG3+miR-NC overexpression group (co-transfected with pcDNA3.1-LncRNA MEG3 and anti-miR-NC), LncRNA MEG3+miR-181a-5p overexpression group (co-transfected with pcDNA 3.1-LncRNA MEG3 and anti-miR-181a-5p), all plasmids were transfected into SiHa cells by liposome method. The cell survival fraction was assessed by colony formation assay. The cell apoptosis rate was evaluated by flow cytometry. The cell fluorescence activity was assessed by dual luciferase reporter assay. The expression levels of PTEN, p-Akt and Akt proteins were detected by Western blot. Results Compared with the radiosensitive group, the expression of LncRNA MEG3 was significantly down-regulated in radiation-resistant cervical cancer tissues (P<0.05), and its expression level was positively correlated with the sensitivity of cervical cancer cells. Overexpression of LncRNA MEG3 or inhibition of miR-181a-5p could significantly enhance the irradiation sensitivity and promote the apoptosis of cervical cancer cell line SiHa (both P<0.05). The fluorescence activity of wild-type LncRNA MEG3 cells was inhibited by miR-181a-5p. Overexpression of miR-181a-5p reversed the irradiation sensitization and pro-apoptosis effect of LncRNA MEG3 and the regulation of the PTEN/Akt signaling pathway on cervical cancer cell. Conclusion LncRNA MEG3 can enhance the sensitivity of cervical cancer cells to radiation exposure, probably by targeting the miR-181a-5p and regulating the PTEN/Akt signaling pathway, which will provide a new direction for improving clinical prognosis of cervical cancer patients.
Fund:Joint Construction Project of Henan Medical Science and Technology Research Plan in 2018(2018020161)
Corresponding Authors:
Liu Zongwen, Email:liuzwhh@sina.com
Cite this article:
Hou Ge,Wang Cheng,Li Ruping et al. LncRAN MEG3 regulates the radiosensitivity of cervical cancer cells by targeting miR-181a-5p[J]. Chinese Journal of Radiation Oncology, 2020, 29(10): 894-900.
Hou Ge,Wang Cheng,Li Ruping et al. LncRAN MEG3 regulates the radiosensitivity of cervical cancer cells by targeting miR-181a-5p[J]. Chinese Journal of Radiation Oncology, 2020, 29(10): 894-900.
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2020, Vol. 29 
