CX43对S24细胞裸鼠脑移植瘤放射敏感性影响

doi:10.3760/cma.j.cn113030-20200104-00013

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Abstract Objective Toinvestigate the impact of connexin 43(CX43) on the connection of S24glioblastoma multiforme (S24-GBM) cellular network and to explore its role on radio-resistance. Methods Specific lentiviral vectors were used to knockout CX43 in S24-GBM stem cells (S24-GBMSCs). Alternatively, carbenoxolone (CBX) was used to block transmission of CX43. Subsequently, the animal subjects grafted with S24-GBMSCs were monitored under a multiphoton laser scanning microscope (MPLSM). Dynamic changes of tumor microtubes (TMs) and transmission of Ca²⁺ and SR101 in the cellular network were recorded. To study the radiosenstivity of S24-GBM before and after CX43 inhibition, MRI scanning of the brains was taken before and after radiation to assess the tumor sizes. Survival time of each subject was also recorded. Results In comparison with control group, knockout of CX43 in S24-GBMSCs led to shorter TMs, less TM connected cells, lower Ca²⁺ synchronicity and SR101 fluorescence, as well as decreased tumor sizes and prolonged survival time (all P<0.01), which were independent from radiation. However, CBX only demonstrated inhibition on the growth of tumors and the diffussion of Ca²⁺ and SR101, without affecting TMs formation. These above-mentioned alterations could be enhanced by the combination of gap43 knockout in S24-GBMSCs with blockage of CX43 by CBX (all P<0.05). Conclusion CX43 plays a critical role in the radioresistance of S24-GBM by influencing the formation of S24-GBM cellular network and the transmission of important signaling molecules including Ca²⁺ and SR101.

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	Articles by authors
	Huang Lulu
	Liu Shengwen
	Zhang Mengxian
	Yu Shiying
	Qiu Hong
	Hu Guoqing

Key words： Radiosensitivity Connexin 43 S24 cell line Transplanted brain tumor in nude mice

Received: 04 January 2020

Fund:Fund programs:National Natural Science Foundation of China (81772680);Double First-class Independent Innovation Discipline Doctor Project of HUST (3011540024, 5001540074, 5001540095);Young and Middle-Aged Medical Backbone Personnel Training Project of Wuhan (2016whzqnyxggrcl)

Corresponding Authors: Yu Shiying, Email:yushiying@medmail.com

Cite this article:

Huang Lulu,Liu Shengwen,Zhang Mengxian et al. Radiosensitivity of connexin 43(CX43) on S24-GBMSCs based brain tumor in nude mice[J]. Chinese Journal of Radiation Oncology, 2020, 29(5): 383-387.

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http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.cn113030-20200104-00013 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2020/V29/I5/383

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