miR-20a通过靶向抑制PTEN诱导肝癌细胞放射抵抗

doi:10.3760/cma.j.issn.1004-4221.2018.09.013

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Abstract

Objective To explore the role and mechanism of miR-20a in the radiosensitivity of hepatocellular carcinoma (HCC). Methods The expression level of miR-20a in HCC cell lines and tissue specimens was detected by real-time fluorescent quantitative PCR.HCC cell line stably over-expressing miR-20a was constructed. The effect of miR-20a on HCC cell radiosensitivity was evaluate by cloning assay. The expression levels of Bcl-2,Caspase-3 and γ-H2AX proteins were quantitatively detected by Western blot. The target gene of the downstream regulation of miR-20a was predicted by bioinformatics analysis,which was further verified by dual luciferase reporter assay,real-time fluorescent quantitative PCR and Western blot. HCC cell line stably overexpressing miR-20a was transfected with pCDNA3.0-PTEN to investigate the changes in the radiosensitivity of cells and to determine whether PTEN is a functional target gene for miR-20a-induced radioresistance of HCC. Results The expression levels of miR-20a was significantly up-regulated in HCC cell line and tissue specimens (both P<0.05).After identical radiotherapy,the cell survival rate,the radioresistance was strengthened (P<0.05),the expression of Bcl-2 was up-regulated,whereas the expression levels of Caspase-3 and γ-H2AX were down-regulated in the LV-miR-20a group compared with those in the blank and control groups (WT and LV-con groups).Overexpression of PTEN could reverse the miR-20a-induced radioresistance. Conclusion miR-20a is up-regulated in the HCC cell lines and tissue specimens. Overexpression of miR-20a can promote the radioresistance of HCC cells. PTEN is a functional target gene for miR-20a-induced radioresistance of HCC,indicating that miR-20a/PTEN site may be an effective molecular target associated with clinical radiotherapy for liver cancer.

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	Articles by authors
	Zhang Yuqin
	Chen Longhua
	Ding Yi
	Zheng Lin

Key words： miR-20a gene PTEN gene Hepatocellular carcinoma cell line Radiosensitivity

Received: 12 August 2017

Fund:

Project of Guangdong Provincial Natural Science Fund(2018030310513); Project of Guangdong Provincial Natural Science Fund(2017A030310081); Project of Guangdong Medical Research Fund(A2016352); The Fundamental Research Funds for the Central Universities(21616333)

Corresponding Authors: Zheng Lin,Email:147938636@qq.com;Ding Yi,Email:460369876@qq.com

Cite this article:

Zhang Yuqin,Chen Longhua,Ding Yi et al. miR-20a induces cell radioresistance by targeting PTEN in hepatocellular carcinoma[J]. Chinese Journal of Radiation Oncology, 2018, 27(9): 850-854.

URL:

http://journal12.magtechjournal.com/Jweb_fszlx/EN/10.3760/cma.j.issn.1004-4221.2018.09.013 OR http://journal12.magtechjournal.com/Jweb_fszlx/EN/Y2018/V27/I9/850

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